Zheng Yueyuan, Liang Yuqing, Zhang Depan, Sun Xiaoyi, Liang Li, Li Juan, Liu You-Nian
College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, P. R. China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
ACS Omega. 2018 May 31;3(5):4766-4775. doi: 10.1021/acsomega.8b00308. Epub 2018 May 1.
Injectable scaffolds are of great interests for skin regeneration because they can fill irregularly shaped defects through minimally invasive surgical treatments. In this study, an injectable hydrogel from biopolymers is developed and its application as wound dressings is examined. Gelatin-based hydrogels were successfully prepared at body temperature upon blending with low content of gellan, and the synergetic effect on the gel formation was carefully characterized through rheological methods. The electrostatic complexation between gelatin and gellan was confirmed to contribute a continuous hydrogel network. The obtained blend hydrogel demonstrates remarkable shear-thinning and self-recovering properties. For antibacterial purpose, tannic acid was incorporated into the blend hydrogel. In addition, tannic acid-loaded blend hydrogel was verified to accelerate the wound healing on the mice model, significantly than the control groups. Thus, this paper presents a facile approach without chemical modification to construct injectable gelatin-based hydrogels, which have great potential as a wound dressing or tissue scaffold at body temperature.
可注射支架对于皮肤再生具有极大的吸引力，因为它们可以通过微创手术治疗来填充形状不规则的缺损。在本研究中，开发了一种基于生物聚合物的可注射水凝胶，并研究了其作为伤口敷料的应用。与低含量的结冷胶混合后，在体温下成功制备了明胶基水凝胶，并通过流变学方法仔细表征了其对凝胶形成的协同作用。明胶与结冷胶之间的静电络合被证实有助于形成连续的水凝胶网络。所获得的共混水凝胶表现出显著的剪切变稀和自我恢复特性。为了达到抗菌目的，将单宁酸掺入共混水凝胶中。此外，经证实，负载单宁酸的共混水凝胶在小鼠模型上比对照组能显著加速伤口愈合。因此，本文提出了一种无需化学修饰的简便方法来构建可注射的明胶基水凝胶，其在体温下作为伤口敷料或组织支架具有巨大潜力。
