State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
Environ Sci Technol. 2012 Jul 17;46(14):7551-7. doi: 10.1021/es301145m. Epub 2012 Jul 2.
We investigated the abundance and distribution of tetracycline resistance genes (tet genes) in an oxytetracycline (OTC) antibiotic production wastewater (APW) treatment system. Of sixteen tet genes and five mobile elements, nine tet genes (tet(A), tet(C), tet(G), tet(L), tet(M), tet(O), tet(Q), tet(W), and tet(X)) and two mobile elements (class 1 integron (intI1) and transposon Tn916/1545) were successfully quantified by real-time PCR. The relative abundance of tet genes in the effluent and activated sludge (1.2 × 10(-4) to 1.3 × 10(0)) of the APW treatment system were up to 2 orders of magnitude higher than those in the OTC fermentation residues (8.5 × 10(-5) to 6.7 × 10(-3)) (P < 0.01), and 1-4 orders of magnitude higher than those in sewage and nonantibiotic production wastewater treatment systems (P < 0.01), showing that the discharge of abundant tet genes from the APW treatment system is worthy of attention. The three most abundant genes in the APW treatment system, tet(A), tet(C), and tet(G), exhibited significant positive correlations with intI1 (R(2) = 0.73, 0.95, and 0.83, respectively; P < 0.05), suggesting that intI1 may be involved in their proliferation. This is the first study showing that some measures may be required to control the discharge of antibiotic resistance genes from treated APW and activated sludge.
我们研究了土霉素（OTC）抗生素生产废水（APW）处理系统中土霉素抗性基因（tet 基因）的丰度和分布。通过实时 PCR 成功定量了 16 个 tet 基因和 5 个移动元件，其中 9 个 tet 基因（tet(A)、tet(C)、tet(G)、tet(L)、tet(M)、tet(O)、tet(Q)、tet(W)和 tet(X)）和 2 个移动元件（类 1 整合子（intI1）和转座子 Tn916/1545）。APW 处理系统中出水和活性污泥（1.2×10(-4)至 1.3×10(0)）中 tet 基因的相对丰度比 OTC 发酵残留物（8.5×10(-5)至 6.7×10(-3)）高 2 个数量级（P < 0.01），比污水和非抗生素生产废水处理系统高 1-4 个数量级（P < 0.01），表明 APW 处理系统中大量 tet 基因的排放值得关注。APW 处理系统中最丰富的三个基因 tet(A)、tet(C)和 tet(G)与 intI1 呈显著正相关（R(2)分别为 0.73、0.95 和 0.83；P < 0.05），表明 intI1 可能参与了它们的增殖。这是首次表明可能需要采取一些措施来控制经处理的 APW 和活性污泥中抗生素抗性基因的排放。
