Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
Molecular and Biological Agricultural Sciences, Taiwan International Graduate Program, National Chung-Hsing University and Academia Sinica, Taipei, Taiwan.
New Phytol. 2012 Sep;195(4):951-961. doi: 10.1111/j.1469-8137.2012.04206.x. Epub 2012 Jun 18.
The direct analysis of phytosiderophores (PSs) and their metal complexes in plants is critical to understanding the biological functions of different PSs. Here we report on a rapid and highly sensitive liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS) method for the direct and simultaneous determination of free PSs and their ferric complexes in plants. In addition to previously reported PSs--deoxymugineic acid (DMA), mugineic acid (MA) and epihydroxymugineic acid (epi-HMA)--two more PSs, avenic acid (AVA) and hydroxyavenic acid (HAVA), were identified by this method in roots of Hordeum vulgare cv Himalaya and in root exudates under iron (Fe) deficiency. The two identified PSs could be responsible for Fe acquisition under Fe deficiency because of their relative abundance and ability to form ferric complexes in secreted root exudates. This LC-ESI-Q-TOF-MS method greatly facilitates the identification of free PSs and PS-Fe complexes in one plant sample.
直接分析植物中的植物铁载体（PSs）及其金属配合物对于理解不同 PSs 的生物学功能至关重要。在这里，我们报告了一种快速且高灵敏度的液相色谱-电喷雾电离-四极杆-飞行时间质谱（LC-ESI-Q-TOF-MS）方法，用于直接和同时测定植物中游离 PSs 和它们的铁配合物。除了先前报道的 PSs——去氧麦根酸（DMA）、麦根酸（MA）和表羟基麦根酸（epi-HMA）——通过这种方法在大麦 cv Himalaya 的根和缺铁条件下的根分泌物中还鉴定出了另外两种 PSs，即燕麦酸（AVA）和羟基燕麦酸（HAVA）。由于这两种鉴定出的 PSs 在分泌的根分泌物中具有相对丰度和形成铁配合物的能力，因此它们可能负责在缺铁条件下获取铁。这种 LC-ESI-Q-TOF-MS 方法极大地方便了在一个植物样品中鉴定游离 PSs 和 PS-Fe 配合物。
