Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742, USA.
Virus Res. 2012 Sep;168(1-2):41-7. doi: 10.1016/j.virusres.2012.06.010. Epub 2012 Jun 16.
As part of our ongoing efforts on animal influenza surveillance in Argentina, an H9N2 virus was isolated from a wild aquatic bird (Netta peposaca), A/rosy-billed pochard/Argentina/CIP051-559/2007 (H9N2) - herein referred to as 559/H9N2. Due to the important role that H9N2 viruses play in the ecology of influenza in nature, the 559/H9N2 isolate was characterized molecularly and biologically. Phylogenetic analysis of the HA gene revealed that the 559/H9N2 virus maintained an independent evolutionary pathway and shared a sister-group relationship with North American viruses, suggesting a common ancestor. The rest of the genome segments clustered with viruses from South America. Experimental inoculation of the 559/H9N2 in chickens and quail revealed efficient replication and transmission only in quail. Our results add to the notion of the unique evolutionary trend of avian influenza viruses in South America. Our study increases our understanding of H9N2 viruses in nature and emphasizes the importance of expanding animal influenza surveillance efforts to better define the ecology of influenza viruses at a global scale.
作为我们在阿根廷进行的动物流感监测工作的一部分，从一只野生水禽（Netta peposaca）中分离到了一株 H9N2 病毒，该病毒被命名为 A/rosy-billed pochard/Argentina/CIP051-559/2007（H9N2）- 以下简称 559/H9N2。由于 H9N2 病毒在自然界流感生态学中起着重要作用，因此对 559/H9N2 分离株进行了分子和生物学特征分析。HA 基因的系统进化分析表明，559/H9N2 病毒保持独立的进化途径，与北美的病毒具有姐妹群关系，提示存在共同的祖先。其余基因组片段与来自南美洲的病毒聚类。559/H9N2 在鸡和鹌鹑中的实验接种表明，该病毒仅在鹌鹑中能够有效复制和传播。我们的研究结果进一步证实了南美洲禽流感病毒独特的进化趋势。本研究增加了我们对自然界中 H9N2 病毒的认识，并强调了扩大动物流感监测工作的重要性，以更好地在全球范围内定义流感病毒的生态学。
