College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Food Chem Toxicol. 2012 Sep;50(9):3215-21. doi: 10.1016/j.fct.2012.06.009. Epub 2012 Jun 16.
The cry1Ac-M gene, coding one of Bacillus thuringiensis (Bt) crystal proteins, was introduced into maize H99 × Hi IIB genome to produce insect-resistant GM maize BT-38. The food safety assessment of the BT-38 maize was conducted in Sprague-Dawley rats by a 90-days feeding study. We incorporated maize grains from BT-38 and H99 × Hi IIB into rodent diets at three concentrations (12.5%, 25%, 50%) and administered to Sprague-Dawley rats (n=10/sex/group) for 90 days. A commercialized rodent diet was fed to an additional group as control group. Body weight, feed consumption and toxicological response variables were measured, and gross as well as microscopic pathology were examined. Moreover, detection of residual Cry1Ac-M protein in the serum of rats fed with GM maize was conducted. No death or adverse effects were observed in the current feeding study. No adverse differences in the values of the response variables were observed between rats that consumed diets containing GM maize BT-38 and non-GM maize H99 × Hi IIB. No detectable Cry1Ac-M protein was found in the serum of rats after feeding diets containing GM maize for 3 months. The results demonstrated that BT-38 maize is as safe as conventional non-GM maize.
Cry1Ac-M 基因，编码苏云金芽孢杆菌（Bt）晶体蛋白之一，被导入玉米 H99×Hi IIB 基因组中，以生产抗虫转基因玉米 BT-38。通过 90 天喂养研究对 BT-38 玉米进行了食品安全评估。我们将 BT-38 和 H99×Hi IIB 的玉米粒分别以三个浓度（12.5%、25%、50%）掺入啮齿动物饲料中，并将其喂给 Sprague-Dawley 大鼠（每组 10 只/性别）90 天。一组以商业化的啮齿动物饲料作为对照组。测量体重、饲料消耗和毒理学反应变量，并检查大体和显微镜病理学。此外，还检测了食用 GM 玉米的大鼠血清中残留的 Cry1Ac-M 蛋白。在本次喂养研究中，未观察到死亡或不良影响。食用含 GM 玉米 BT-38 和非 GM 玉米 H99×Hi IIB 的大鼠的反应变量值没有观察到不良差异。在食用含 GM 玉米 3 个月后，大鼠血清中未检测到可检测的 Cry1Ac-M 蛋白。结果表明，BT-38 玉米与常规非 GM 玉米一样安全。
