Institute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163, Berlin, Germany.
Department of Liquor Making Engineering, Moutai Institute, Luban Avenue, 564507, Renhuai, China.
Sci Rep. 2019 Dec 12;9(1):18915. doi: 10.1038/s41598-019-55375-5.
In a recent one-year feeding study, we observed no adverse effects on tissue level in organs of rats fed with the genetically-modified maize MON810. Here, we assessed RNA expression levels of 86 key genes of the apoptosis-, NF-кB-, DNA-damage response (DDR)-, and unfolded-protein response (UPR) pathways by RT-qPCR in the rat liver. Male and female rats were fed either with 33% MON810 (GMO), isogenic- (ISO), or conventional maize (CONV) and RNAs were quantified from eight rats from each of the six feeding groups. Only Birc2 transcript showed a significant (p ≤ 0.05) consistent difference of ≥1.5-fold between the GMO and ISO groups in both sexes. Unsupervised cluster analysis showed a strong separation of male and female rats, but no clustering of the feeding groups. Individual analysis of the pathways did not show any clustering of the male or female feeding groups either, though transcript levels of UPR pathway-associated genes caused some clustering of the male GMO and CONV feeding group samples. These differences were not seen between the GMO and ISO control or within the female cohort. Our data therefore does not support an adverse effect on rat liver RNA expression through the long-term feeding of MON810 compared to isogenic control maize.
在最近的一项为期一年的喂养研究中，我们观察到喂食转基因玉米 MON810 的大鼠的组织器官未出现不良反应。在此，我们通过 RT-qPCR 评估了大鼠肝脏中凋亡、NF-кB、DNA 损伤反应 (DDR) 和未折叠蛋白反应 (UPR) 途径中 86 个关键基因的 RNA 表达水平。雄性和雌性大鼠分别喂食 33%的 MON810（GMO）、同基因（ISO）或常规玉米（CONV），从六个喂养组的每一组中各取 8 只大鼠定量 RNA。仅 Birc2 转录本在两性中均表现出显著（p≤0.05）的一致差异，GMO 和 ISO 组之间的差异≥1.5 倍。非监督聚类分析显示，雄性和雌性大鼠之间存在强烈的分离，但喂养组之间没有聚类。对各途径的单独分析也没有显示出雄性或雌性喂养组的任何聚类，尽管 UPR 途径相关基因的转录本水平导致雄性 GMO 和 CONV 喂养组样本的一些聚类。在 GMO 和 ISO 对照或雌性队列中均未观察到这些差异。因此，与同基因对照玉米相比，长期喂食 MON810 对大鼠肝脏 RNA 表达没有不利影响。
