South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang, Guangdong, 524091, China.
Gene. 2012 Sep 1;505(2):368-73. doi: 10.1016/j.gene.2012.06.011. Epub 2012 Jun 16.
FLOWERING LOCUS T (FT)-like genes are crucial regulators of flowering in angiosperms. A homolog of FT, designated as AcFT (GenBank ID: HQ343233), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcFT is 915 bp in length and contains an ORF of 534 bp, which encodes a protein of 177 aa. Molecular weight was 19.9 kDa and isoelectric point was 6.96. The deduced protein sequence of AcFT was 84% and 82% identical to homologs encoded by CgFT in Cymbidium goeringii and OgFT in Oncidium Gower Ramsey respectively. Quantitative real-time PCR (qRT-PCR) analyses showed that the expression of AcFT was high in flesh and none in leaves. qRT-PCR analyses in different stages indicated that the expression of AcFT reached the highest level on 40 d after flower inducing, when the multiple fruit and floral organs were forming. The 35S::AcFT transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.
FT 同源基因是开花植物开花的关键调控因子。通过反转录聚合酶链反应（RT-PCR）和快速扩增 cDNA 末端（RACE），从菠萝品种 Comte de Paris 中分离出一个 FT 同源基因，命名为 AcFT（GenBank ID：HQ343233）。AcFT 的 cDNA 序列长 915bp，包含一个 534bp 的开放阅读框，编码一个 177 个氨基酸的蛋白质。分子量为 19.9kDa，等电点为 6.96。AcFT 的推导蛋白序列与 Cymbidium goeringii 中的 CgFT 和 Oncidium Gower Ramsey 中的 OgFT 编码的同源物分别具有 84%和 82%的同一性。定量实时 PCR（qRT-PCR）分析表明，AcFT 在果肉中的表达量较高，而在叶片中则没有表达。不同阶段的 qRT-PCR 分析表明，在诱导开花后 40d，当多个果实和花器官形成时，AcFT 的表达水平达到最高。35S::AcFT 转基因拟南芥比野生型植物开花更早，花序或枝条更多。
