• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于柑橘叶斑病毒的病毒载体诱导幼年柑橘早熟开花：遗传学和育种的新工具

Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding.

作者信息

Velázquez Karelia, Agüero Jesús, Vives María C, Aleza Pablo, Pina José A, Moreno Pedro, Navarro Luis, Guerri José

机构信息

Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain.

出版信息

Plant Biotechnol J. 2016 Oct;14(10):1976-85. doi: 10.1111/pbi.12555. Epub 2016 Mar 25.

DOI:10.1111/pbi.12555
PMID:26920394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5043495/
Abstract

The long juvenile period of citrus trees (often more than 6 years) has hindered genetic improvement by traditional breeding methods and genetic studies. In this work, we have developed a biotechnology tool to promote transition from the vegetative to the reproductive phase in juvenile citrus plants by expression of the Arabidopsis thaliana or citrus FLOWERING LOCUS T (FT) genes using a Citrus leaf blotch virus-based vector (clbvINpr-AtFT and clbvINpr-CiFT, respectively). Citrus plants of different genotypes graft inoculated with either of these vectors started flowering within 4-6 months, with no alteration of the plant architecture, leaf, flower or fruit morphology in comparison with noninoculated adult plants. The vector did not integrate in or recombine with the plant genome nor was it pollen or vector transmissible, albeit seed transmission at low rate was detected. The clbvINpr-AtFT is very stable, and flowering was observed over a period of at least 5 years. Precocious flowering of juvenile citrus plants after vector infection provides a helpful and safe tool to dramatically speed up genetic studies and breeding programmes.

摘要

柑橘树较长的幼年期（通常超过6年）阻碍了通过传统育种方法进行的遗传改良和遗传研究。在这项工作中，我们开发了一种生物技术工具，通过使用基于柑橘叶斑病毒的载体（分别为clbvINpr-AtFT和clbvINpr-CiFT）表达拟南芥或柑橘的成花素基因（FT），来促进幼年柑橘植株从营养生长阶段向生殖生长阶段转变。用这些载体中的任何一种进行嫁接接种的不同基因型柑橘植株在4 - 6个月内开始开花，与未接种的成年植株相比，其植株结构、叶片、花朵或果实形态均未改变。该载体未整合到植物基因组中，也未与植物基因组重组，并且既不能通过花粉传播，也不能通过载体传播，不过检测到有低频率的种子传播。clbvINpr-AtFT非常稳定，在至少5年的时间里都能观察到开花现象。载体感染后幼年柑橘植株的早熟开花为大幅加速遗传研究和育种计划提供了一个有用且安全的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/59cbbdd8d145/PBI-14-1976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/aee6e6e9f014/PBI-14-1976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/f852db243353/PBI-14-1976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/79d5a0790ff4/PBI-14-1976-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/f238481c8d18/PBI-14-1976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/5c0e92dcb50f/PBI-14-1976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/f3130a44dd0e/PBI-14-1976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/80a10be2d3cc/PBI-14-1976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/59cbbdd8d145/PBI-14-1976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/aee6e6e9f014/PBI-14-1976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/f852db243353/PBI-14-1976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/79d5a0790ff4/PBI-14-1976-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/f238481c8d18/PBI-14-1976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/5c0e92dcb50f/PBI-14-1976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/f3130a44dd0e/PBI-14-1976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/80a10be2d3cc/PBI-14-1976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/11389060/59cbbdd8d145/PBI-14-1976-g005.jpg

相似文献

1
Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding.基于柑橘叶斑病毒的病毒载体诱导幼年柑橘早熟开花：遗传学和育种的新工具
Plant Biotechnol J. 2016 Oct;14(10):1976-85. doi: 10.1111/pbi.12555. Epub 2016 Mar 25.
2
Apple latent spherical virus vector-induced flowering for shortening the juvenile phase in Japanese gentian and lisianthus plants.苹果潜隐球形病毒载体诱导日本龙胆和洋桔梗植株开花以缩短幼年期
Planta. 2016 Jul;244(1):203-14. doi: 10.1007/s00425-016-2498-2. Epub 2016 Mar 25.
3
Reduced generation time of apple seedlings to within a year by means of a plant virus vector: a new plant-breeding technique with no transmission of genetic modification to the next generation.利用植物病毒载体将苹果实生苗的培育时间缩短至一年以内：一种新型的植物育种技术，不会将遗传修饰传递给下一代。
Plant Biotechnol J. 2014 Jan;12(1):60-8. doi: 10.1111/pbi.12116. Epub 2013 Sep 3.
4
Expression of FLOWERING LOCUS T from Arabidopsis thaliana induces precocious flowering in soybean irrespective of maturity group and stem growth habit.拟南芥 FLOWERING LOCUS T 的表达导致大豆提前开花，而与成熟组和茎生长习性无关。
Planta. 2011 Mar;233(3):561-8. doi: 10.1007/s00425-010-1318-3. Epub 2010 Nov 30.
5
The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions.血管靶向柑橘 FLOWERING LOCUS T3 基因促进 Carrizo 砧木和嫁接幼年接穗的非诱导性早期开花。
Sci Rep. 2020 Dec 8;10(1):21404. doi: 10.1038/s41598-020-78417-9.
6
Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector.利用苹果潜隐球形病毒载体中外源表达拟南芥 FT 基因促进苹果实生苗开花和缩短世代时间。
Plant Mol Biol. 2011 Jan;75(1-2):193-204. doi: 10.1007/s11103-010-9718-0. Epub 2010 Dec 5.
7
Virus-induced overexpression of heterologous FLOWERING LOCUS T for efficient speed breeding in tomato.病毒诱导的异源 FLOWERING LOCUS T 的过表达可提高番茄的快速繁殖效率。
J Exp Bot. 2024 Jan 1;75(1):36-44. doi: 10.1093/jxb/erad369.
8
Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants.基于柑橘碎叶病毒的病毒载体诱导的基因沉默在本氏烟和柑橘植物中的效果不同。
Virology. 2014 Jul;460-461:154-64. doi: 10.1016/j.virol.2014.04.017. Epub 2014 Jun 4.
9
Abscisic acid affects expression of citrus FT homologs upon floral induction by low temperature in Satsuma mandarin (Citrus unshiu Marc.).脱落酸在低温诱导温州蜜柑成花过程中影响柑橘 FT 同源物的表达。
Tree Physiol. 2018 May 1;38(5):755-771. doi: 10.1093/treephys/tpx145.
10
FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus.FT基因的过表达诱导桉树早熟开花和正常的生殖发育。
Plant Biotechnol J. 2016 Feb;14(2):808-19. doi: 10.1111/pbi.12431. Epub 2015 Jul 1.

引用本文的文献

1
Virus-Induced Gene Silencing to Unravel the Function of Nicotiana benthamiana Genes Linked to Corolla Abscission.利用病毒诱导基因沉默技术解析与烟草花冠脱落相关的本氏烟草基因的功能
Methods Mol Biol. 2025;2916:139-151. doi: 10.1007/978-1-0716-4470-6_13.
2
Taming the wild: domesticating untapped northern fruit tree and shrub resources in the era of high-throughput technologies.驯服野生植物：在高通量技术时代驯化未开发的北方果树和灌木资源。
AoB Plants. 2025 Jan 4;17(1):plae074. doi: 10.1093/aobpla/plae074. eCollection 2025 Jan.
3
Molecular mechanisms of flowering phenology in trees.

本文引用的文献

1
Seed Transmission of Citrus leaf botch virus: Implications in Quarantine and Certification Programs.柑橘叶斑病毒的种子传播：对检疫和认证计划的影响。
Plant Dis. 2004 Aug;88(8):906. doi: 10.1094/PDIS.2004.88.8.906C.
2
Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants.基于柑橘碎叶病毒的病毒载体诱导的基因沉默在本氏烟和柑橘植物中的效果不同。
Virology. 2014 Jul;460-461:154-64. doi: 10.1016/j.virol.2014.04.017. Epub 2014 Jun 4.
3
Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication.
树木开花物候的分子机制
For Res (Fayettev). 2023 Jan 16;3:2. doi: 10.48130/FR-2023-0002. eCollection 2023.
4
Genome-wide identification and characterization of flowering genes in Citrus sinensis (L.) Osbeck: a comparison among C. Medica L., C. Reticulata Blanco, C. Grandis (L.) Osbeck and C. Clementina.柑橘属植物开花基因的全基因组鉴定和特征分析：比较药用柑橘、红橘、甜橙和克莱门氏小柑橘。
BMC Genom Data. 2024 Feb 20;25(1):20. doi: 10.1186/s12863-024-01201-5.
5
Advancing tree genomics to future proof next generation orchard production.推动树木基因组学发展，为下一代果园生产提供未来保障。
Front Plant Sci. 2024 Jan 19;14:1321555. doi: 10.3389/fpls.2023.1321555. eCollection 2023.
6
Promoter insertion leads to polyembryony in mango - a case of convergent evolution with citrus.启动子插入导致芒果多胚现象——与柑橘趋同进化的一个例子。
Hortic Res. 2023 Nov 8;10(12):uhad227. doi: 10.1093/hr/uhad227. eCollection 2023 Dec.
7
CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding.CRISPR/Cas 作为一种基因组编辑技术在果树育种中的应用。
Int J Mol Sci. 2023 Nov 23;24(23):16656. doi: 10.3390/ijms242316656.
8
Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation.利用 Cas12a/crRNA 核糖核蛋白在 T0 代中生成无转基因溃疡抗性柑橘。
Nat Commun. 2023 Jul 5;14(1):3957. doi: 10.1038/s41467-023-39714-9.
9
Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry ().鉴定和表征来源于桑树的新型泉州桑树病毒
Viruses. 2023 May 9;15(5):1131. doi: 10.3390/v15051131.
10
Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus.基于测序的基因分型用于基于单核苷酸多态性的连锁分析以及柑橘雄性不育和多胚性的竞争性等位基因特异性PCR（KASPar）标记开发
Plants (Basel). 2023 Apr 6;12(7):1567. doi: 10.3390/plants12071567.
对不同的橘、柚和甜橙基因组进行测序，揭示了柑橘驯化过程中复杂的杂交历史。
Nat Biotechnol. 2014 Jul;32(7):656-62. doi: 10.1038/nbt.2906. Epub 2014 Jun 8.
4
Structural features determining flower-promoting activity of Arabidopsis FLOWERING LOCUS T.决定拟南芥 FLOWERING LOCUS T 花诱导活性的结构特征。
Plant Cell. 2014 Feb;26(2):552-64. doi: 10.1105/tpc.113.115220. Epub 2014 Feb 14.
5
Genetically based location from triploid populations and gene ontology of a 3.3-mb genome region linked to Alternaria brown spot resistance in citrus reveal clusters of resistance genes.基于三倍体群体的遗传定位和与柑橘炭疽病抗性相关的 3.3-Mb 基因组区域的基因本体论揭示了抗性基因簇。
PLoS One. 2013 Oct 8;8(10):e76755. doi: 10.1371/journal.pone.0076755. eCollection 2013.
6
Reduced generation time of apple seedlings to within a year by means of a plant virus vector: a new plant-breeding technique with no transmission of genetic modification to the next generation.利用植物病毒载体将苹果实生苗的培育时间缩短至一年以内：一种新型的植物育种技术，不会将遗传修饰传递给下一代。
Plant Biotechnol J. 2014 Jan;12(1):60-8. doi: 10.1111/pbi.12116. Epub 2013 Sep 3.
7
Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus.柑橘碎叶病毒侵染本氏烟和柑橘的分生组织区域。
Mol Plant Pathol. 2013 Aug;14(6):610-6. doi: 10.1111/mpp.12031. Epub 2013 Apr 8.
8
A reference genetic map of C. clementina hort. ex Tan.; citrus evolution inferences from comparative mapping.甜橙参考遗传图谱；基于比较作图的柑橘进化推断。
BMC Genomics. 2012 Nov 5;13:593. doi: 10.1186/1471-2164-13-593.
9
Isolation and characterization of a FLOWERING LOCUS T homolog from pineapple (Ananas comosus (L.) Merr).菠萝（凤梨属 Ananas comosus (L.) Merr.）开花同源异型基因 FLO 的分离与鉴定。
Gene. 2012 Sep 1;505(2):368-73. doi: 10.1016/j.gene.2012.06.011. Epub 2012 Jun 16.
10
Development of viral vectors based on Citrus leaf blotch virus to express foreign proteins or analyze gene function in citrus plants.基于柑橘碎叶病毒的病毒载体的开发，用于在柑橘植物中表达外源蛋白或分析基因功能。
Mol Plant Microbe Interact. 2012 Oct;25(10):1326-37. doi: 10.1094/MPMI-02-12-0048-R.