Zhao Lihua, Liu Liping, Liu Yanhui, Dou Xianying, Cai Hanyang, Aslam Mohammad, Hou Zhimin, Jin Xingyue, Li Yi, Wang Lulu, Zhao Heming, Wang Xiaomei, Sicard Adrien, Qin Yuan
College of Life Science, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China.
Department of Plant Biology, Swedish University of Agricultural Sciences, Uppsala BioCenter and Linnean Centre for Plant Biology, Uppsala, Sweden.
Hortic Res. 2021 Nov 1;8(1):239. doi: 10.1038/s41438-021-00669-x.
Understanding germline specification in plants could be advantageous for agricultural applications. In recent decades, substantial efforts have been made to understand germline specification in several plant species, including Arabidopsis, rice, and maize. However, our knowledge of germline specification in many agronomically important plant species remains obscure. Here, we characterized the female germline specification and subsequent female gametophyte development in pineapple using callose staining, cytological, and whole-mount immunolocalization analyses. We also determined the male germline specification and gametophyte developmental timeline and observed male meiotic behavior using chromosome spreading assays. Furthermore, we identified 229 genes that are preferentially expressed at the megaspore mother cell (MMC) stage during ovule development and 478 genes that are preferentially expressed at the pollen mother cell (PMC) stage of anther development using comparative transcriptomic analysis. The biological functions, associated regulatory pathways and expression patterns of these genes were also analyzed. Our study provides a convenient cytological reference for exploring pineapple germline development and a molecular basis for the future functional analysis of germline specification in related plant species.
了解植物中的生殖细胞特化对于农业应用可能具有优势。近几十年来，人们为了解包括拟南芥、水稻和玉米在内的几种植物物种中的生殖细胞特化付出了巨大努力。然而，我们对许多具有重要农艺价值的植物物种中的生殖细胞特化的了解仍然模糊不清。在这里，我们使用胼胝质染色、细胞学和整装免疫定位分析，对菠萝中的雌性生殖细胞特化及随后的雌配子体发育进行了表征。我们还确定了雄性生殖细胞特化和配子体发育时间表，并使用染色体铺展分析观察了雄性减数分裂行为。此外，我们通过比较转录组分析，鉴定出229个在胚珠发育的大孢子母细胞（MMC）阶段优先表达的基因，以及478个在花药发育的花粉母细胞（PMC）阶段优先表达的基因。还分析了这些基因的生物学功能、相关调控途径和表达模式。我们的研究为探索菠萝生殖细胞发育提供了便利的细胞学参考，并为未来相关植物物种中生殖细胞特化的功能分析提供了分子基础。
