School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
Neurochem Int. 2012 Jun;60(8):827-36. doi: 10.1016/j.neuint.2012.03.012. Epub 2012 Mar 28.
Tianma (Rhizoma gastrodiae) is the dried rhizome of the plant Gastrodia elata Blume (Orchidaceae family). As a medicinal herb in traditional Chinese medicine (TCM) its functions are to control convulsions, pain, headache, dizziness, vertigo, seizure, epilepsy and others. In addition, tianma is frequently used for the treatment of neurodegenerative disorders though the mechanism of action is widely unknown. Accordingly, this study was designed to examine the effects of tianma on the proteome metabolism in differentiated human neuronal SH-SY5Y cells to explore its specific effects on neuronal signaling pathways. Using an iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomics research approach, we identified 2390 modulated proteins, out of which 406 were found to be altered by tianma in differentiated human neuronal SH-SY5Y cells. Based on the observed data, we hypothesize that tianma promotes neuro-regenerative signaling cascades by controlling chaperone/proteasomal degradation pathways (e.g. CALR, FKBP3/4, HSP70/90) and mobilizing neuro-protective genes (such as AIP5) as well as modulating other proteins (RTN1/4, NCAM, PACSIN2, and PDLIM1/5) with various regenerative modalities and capacities related to neuro-synaptic plasticity.
天麻（Rhizoma gastrodiae）是兰科植物天麻（Gastrodia elata Blume）的干燥根茎。作为传统中药（TCM）中的一种草药，其功能是控制惊厥、疼痛、头痛、头晕、眩晕、抽搐、癫痫等。此外，天麻还常用于治疗神经退行性疾病，尽管其作用机制尚不清楚。因此，本研究旨在研究天麻对分化人神经元 SH-SY5Y 细胞蛋白质组代谢的影响，以探讨其对神经元信号通路的具体作用。本研究采用基于 iTRAQ（相对和绝对定量同位素标记）的蛋白质组学研究方法，共鉴定出 2390 个受调控的蛋白质，其中 406 个在分化的人神经元 SH-SY5Y 细胞中被天麻改变。根据观察到的数据，我们假设天麻通过控制伴侣蛋白/蛋白酶体降解途径（如 CALR、FKBP3/4、HSP70/90）和动员神经保护基因（如 AIP5）以及调节其他蛋白质（RTN1/4、NCAM、PACSIN2 和 PDLIM1/5），从而促进神经再生信号级联反应，这些蛋白质具有与神经突触可塑性相关的各种再生方式和能力。
