Dipartimento Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy.
J Exp Zool B Mol Dev Evol. 2012 Jul;318(5):404-16. doi: 10.1002/jez.b.22455.
The coelacanth Latimeria menadoensis, a living fossil, occupies a key phylogenetic position to explore the changes that have affected the genomes of the aquatic vertebrates that colonized dry land. This is the first study to isolate and analyze L. menadoensis mRNA. Three different vitellogenin transcripts were identified and their inferred amino acid sequences compared to those of other known vertebrates. The phylogenetic data suggest that the evolutionary history of this gene family in coelacanths was characterized by a different duplication event than those which occurred in teleosts, amniotes, and amphibia. Comparison of the three sequences highlighted differences in functional sites. Moreover, despite the presence of conserved sites compared with the other oviparous vertebrates, some sites were seen to have changed, others to be similar only to those of teleosts, and others still to resemble only to those of tetrapods.
被称为活化石的矛尾鱼（Latimeria menadoensis），在探讨影响水生脊椎动物向陆地移居的基因组变化方面，处于关键的进化位置。这是首次对矛尾鱼的 mRNA 进行分离和分析。本文鉴定了三种不同的卵黄蛋白原转录本，并对其推断的氨基酸序列与其他已知脊椎动物的序列进行了比较。系统发育数据分析表明，与硬骨鱼、羊膜动物和两栖动物相比，软骨鱼卵黄蛋白原基因家族的进化历史具有不同的重复事件特征。对这三个序列的比较突出了功能位点的差异。此外，尽管与其他卵生脊椎动物相比存在保守位点，但有些位点发生了变化，有些位点仅与硬骨鱼相似，还有些位点与四足动物相似。
