Rahbari Amir Hossien, Keshavarz Hossien, Shojaee Saeedeh, Mohebali Mehdi, Rezaeian Mostafa
Tehran University of Medical Sciences, Tehran, Iran.
Korean J Parasitol. 2012 Jun;50(2):99-102. doi: 10.3347/kjp.2012.50.2.99. Epub 2012 May 24.
Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI ≤ 50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.
从伊朗德黑兰的不同实验室收集了总共100份血清样本，并使用间接免疫荧光抗体试验（IFAT）检测抗弓形虫特异性IgG和IgM抗体。使用IgG（慢性）和IgM（急性）阳性样本，在96孔微量滴定板的双行中通过ELISA进行IgG亲和力试验。一行用6M尿素洗涤，另一行用PBS（pH 7.2）洗涤，然后计算亲和力指数（AI）。18份急性弓形虫病血清中有16份（88.9%）显示低亲和力水平（AI≤50），82份感染慢性期血清中有76份（92.7%）显示高亲和力指数（AI>60）。6份血清的AI处于临界范围。结果表明，ELISA法检测IgG亲和力可区分人类弓形虫病的急性和慢性阶段。
