Sharifi Karam, Hosseini Farash Bibi Razieh, Tara Fatemeh, Khaledi Azad, Sharifi Karim, Shamsian Seyed Ali Akbar
Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Research Center of Skin Diseases and Cutaneous Leishmaniasis, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Parasitol. 2019 Oct-Dec;14(4):639-645.
We aimed to evaluate the diagnosis of acute toxoplasmosis by IgG avidity test in pregnant women.
In this cross-sectional study, 250 blood samples were collected from pregnant women with the first month of their pregnancy referring to health centers of University in Mashhad during 2016. Samples were centrifuged at 3000 rpm for 5 min for separation of serum and were kept in the -20 until use. To detection of acute and chronic toxoplasmosis, anti- antibodies (IgG and IgM, and IgG avidity tests were performed using ELISA. Then, data analyzed using SPSS software by Frequency, Pearson Chi-Square, Likelihood Ratio, and Exact tests. And <0.05 was statistically considered as significant.
Total prevalence of IgG and IgM was 23.2% and 7.2%, respectively. A significant correlation was observed between the mean age and IgG level (<0.05). It was not found any correlation between the history of raw meat consumption, cats keeping, education, and residency site. Moreover, 16 people (6.4%) had IgM antibody, of which, 10 cases (62.5%) with low avidity for IgG and 1 people (6.2%) with moderate avidity and 5 cases (31.3%) with high avidity for IgG. Moreover, 76% of pregnant women were seronegative.
More than half of the women (62.5%) with positive IgM antibody in their serum had a low avidity for IgG which revealed an acute infection among pregnant women. infection should be considered as an important factor that affects the pregnancy and IgG avidity as an important test for screening the women who need the treatment.
我们旨在通过IgG亲和力试验评估孕妇急性弓形虫病的诊断。
在这项横断面研究中，2016年期间从马什哈德大学健康中心就诊的怀孕首月孕妇中采集了250份血样。样本以3000转/分钟的速度离心5分钟以分离血清，并保存在-20℃直至使用。为检测急性和慢性弓形虫病，使用酶联免疫吸附测定法进行抗弓形虫抗体（IgG和IgM）及IgG亲和力试验。然后，使用SPSS软件通过频率、Pearson卡方检验、似然比检验和确切概率法对数据进行分析。P<0.05被视为具有统计学意义。
IgG和IgM的总患病率分别为23.2%和7.2%。观察到平均年龄与IgG水平之间存在显著相关性（P<0.05）。未发现食用生肉史、养猫、教育程度和居住地点之间存在相关性。此外，16人（6.4%）有IgM抗体，其中10例（62.5%）对IgG亲和力低，1人（6.2%）对IgG亲和力中等，5例（31.3%）对IgG亲和力高。此外，76%的孕妇血清学阴性。
血清中IgM抗体阳性的女性中，超过一半（62.5%）对IgG亲和力低，这表明孕妇存在急性感染。感染应被视为影响妊娠的重要因素，而IgG亲和力作为筛选需要治疗的女性的重要检测方法。
