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[新疆特殊生境石内细菌群落的末端限制性片段长度多态性分析]

[Terminal restriction fragment length polymorphism analysis of endolithic bacteria community at special habitats in Xinjiang].

作者信息

Mu Wenting, Zhang Tao, Sun Jian, Zeng Jun, Xu YingHua, Wu Zunfeng, Hu Wen, Lou Kai

机构信息

College of Life Science and Technology of Xinjiang University, Urumqi 830046, China.

出版信息

Wei Sheng Wu Xue Bao. 2012 Mar 4;52(3):381-8.

PMID:22712410
Abstract

OBJECTIVE

In order to investigate the composition and diversity of endolithic bacteria at special habitats in Xinjiang.

METHODS

Five rock samples were collected, including Wusu's granite (sample 1), Glacier No. 1, and Mulei's metamorphic rock (sample 2, sample 3), Yumin and Tokesun's Rock varnish (sample 4, sample 5). Endolithic bacterial community composition and diversity were analyzed by the method of Terminal Restriction Fragment Length Polymorphism.

RESULTS

Differences in diversity indexes among samples were not apparent. Clustering analysis suggested that similarity coefficient was higher in same rock type, sample 2 and sample 3 were grouped together, then sample 1 clustered with them, and sample 4 and sample 5 were classified together. All samples harbored these phyla such as Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes. Acidobacteria and Planctomycetes existed in sample 1 and sample 2, respectively; Sample 5 was dominated by Actinobacteria, while other samples were dominated by Proteobacteria.

CONCLUSION

The endolithic bacterial composition of same rock type collected at various habitats was different. Meanwhile, a diversity of novel species and lineages maybe existed in rocks.

摘要

目的

为了研究新疆特殊生境下石内细菌的组成和多样性。

方法

采集了5个岩石样本,包括乌苏的花岗岩(样本1)、一号冰川以及木垒的变质岩(样本2、样本3)、裕民和托克逊的岩石漆(样本4、样本5)。采用末端限制性片段长度多态性方法分析石内细菌群落组成和多样性。

结果

样本间多样性指数差异不明显。聚类分析表明,同一岩石类型的相似系数较高,样本2和样本3聚在一起,然后样本1与它们聚类,样本4和样本5归为一类。所有样本均含有厚壁菌门、放线菌门、变形菌门和拟杆菌门等菌门。酸杆菌门和浮霉菌门分别存在于样本1和样本2中;样本5以放线菌门为主,而其他样本以变形菌门为主。

结论

在不同生境采集的同一岩石类型的石内细菌组成不同。同时,岩石中可能存在多种新物种和谱系。

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