Department of Physical Education, São Paulo State University - UNESP, Av: 24-A, 1515 Bela Vista,, 13506-900, Rio Claro, São Paulo, Brazil.
Lipids Health Dis. 2012 Jun 19;11:78. doi: 10.1186/1476-511X-11-78.
The main purpose of this research was to investigate the alterations in the aerobic capacity and appearance of metabolic alterations in Wistar rats fed on fructose-rich diet. We separated twenty-eight rats into two groups according to diet: a control group (C) (balanced diet) and a fructose-rich diet group (F). The animals were fed these diets for 60 d (d 120 to 180). We performed insulin, glucose as well as a minimum lactate test, at d 120 and 180. At the end of the experiment, sixteen animals were euthanized, and the following main variables were analysed: aerobic capacity, the serum aspartate aminotransferase (AST) to alanine aminotransferase (ALT) ratio, serum and liver triglyceride concentrations, serum and liver thiobarbituric acid reactive substance (TBARS) concentrations, serum and liver catalase and superoxide dismutase (SOD) activity and haematoxylin-eosin histology (HE) in hepatocytes. The remaining twelve animals were submitted to an analysis of their hepatic lipogenic rate. The animals fed a fructose-rich diet exhibited a reduction in aerobic capacity, glucose tolerance and insulin sensitivity and increased concentrations of triglycerides and TBARS in the liver. Catalase and SOD activities were reduced in the livers of the fructose-fed animals. In addition, the serum AST/ALT ratio was higher than that of the C group, which indicates hepatic damage, and the damage was confirmed by histology. In conclusion, the fructose-rich diet caused significant liver damage and a reduction in insulin sensitivity in the animals, which could lead to deleterious metabolic effects.
这项研究的主要目的是研究喂食富含果糖的饮食对 Wistar 大鼠有氧能力和代谢变化外观的影响。我们根据饮食将 28 只大鼠分为两组：对照组（C）（均衡饮食）和富含果糖饮食组（F）。这些动物喂食这些饮食 60 天（第 120 天至第 180 天）。我们在第 120 天和第 180 天进行了胰岛素、葡萄糖以及最小乳酸测试。实验结束时，16 只动物被安乐死，分析了以下主要变量：有氧能力、血清天冬氨酸氨基转移酶（AST）与丙氨酸氨基转移酶（ALT）比值、血清和肝脏甘油三酯浓度、血清和肝脏硫代巴比妥酸反应物（TBARS）浓度、血清和肝脏过氧化氢酶和超氧化物歧化酶（SOD）活性以及肝细胞苏木精-伊红组织学（HE）。其余 12 只动物接受了肝脂生成率分析。喂食富含果糖的饮食的动物表现出有氧能力、葡萄糖耐量和胰岛素敏感性降低，以及肝脏甘油三酯和 TBARS 浓度增加。果糖喂养动物的肝脏过氧化氢酶和 SOD 活性降低。此外，血清 AST/ALT 比值高于 C 组，表明肝损伤，组织学检查证实了这一点。总之，富含果糖的饮食导致动物肝脏严重损伤和胰岛素敏感性降低，可能导致有害的代谢影响。
