School of Marine Science and Technology, Huaihai Institute of Technology, 59 Changwu Road, Lianyungang, 222005, China.
Fish Physiol Biochem. 2012 Dec;38(6):1815-1824. doi: 10.1007/s10695-012-9678-1. Epub 2012 Jun 20.
In this study, we investigated the gross structure, secondary structure, and antitumor and mitogenic activity of GANL, a lectin from the gill of bighead carp (Aristichthys nobilis). We used infrared spectroscopy, β-elimination, and circular dichroism spectroscopy to determine the structure of GANL. We measured antiproliferation activity against six human tumor cell lines and mitogenic activity against murine splenocytes using the MTT assay. Based on infrared spectroscopy and β-elimination, we conclude that GANL is a glycoprotein. The protein and carbohydrate moieties are joined by O-glycosidic linkage. A circular dichroism spectroscopic analysis revealed that the secondary structure of GANL consists of α-helices (34.8 %), β-sheets (12.1 %), β-turns (24.5 %), and unordered structures (33.0 %). GANL exerted potent antitumor activity against the HeLa cell line (IC(50) = 11.86 μg/mL) and a mitogenic effect on murine splenocytes in the MTT assay. GANL, a lectin that is isolated from the gills of bighead carp, is a glycoprotein with potent antitumor and mitogenic activity.
在这项研究中，我们研究了从鳙鱼（Aristichthys nobilis）鳃中分离得到的凝集素 GANL 的大体结构、二级结构以及抗肿瘤和有丝分裂原活性。我们使用红外光谱、β-消除和圆二色性光谱来确定 GANL 的结构。我们使用 MTT 法测定了其对六种人肿瘤细胞系的增殖抑制活性和对小鼠脾细胞的有丝分裂原活性。基于红外光谱和 β-消除实验，我们得出结论，GANL 是一种糖蛋白。蛋白质和碳水化合物部分通过 O-糖苷键连接。圆二色性光谱分析表明，GANL 的二级结构由α-螺旋（34.8％）、β-折叠（12.1％）、β-转角（24.5％）和无规卷曲（33.0％）组成。GANL 对 HeLa 细胞系表现出很强的抗肿瘤活性（IC50 = 11.86 μg/mL），并在 MTT 测定中对小鼠脾细胞具有有丝分裂原活性。GANL 是一种从鳙鱼鳃中分离得到的凝集素，是一种具有抗肿瘤和有丝分裂原活性的糖蛋白。
