Halling A, Forsberg J G
Department of Anatomy, University of Lund, Sweden.
Biol Reprod. 1990 Sep;43(3):472-7. doi: 10.1095/biolreprod43.3.472.
Inbred and random-bred NMRI mice were treated with diethylstilbestrol (DES, 5 micrograms per day) or vehicle (olive oil) on Days 1-5 after birth. At the age of 8 wk, females were treated with saline or eCG and hCG to induce ovulation. Ova never occurred in the ampulla of the uterine tube of saline-treated, DES-treated females when these mice were not mated. After gonadotropin treatment, ova were found in the ampulla of all olive oil-treated females and in approximately 80% of DES-treated females. The number of ovulated ova was similar in both groups. Twenty percent of gonadotropin-treated, DES-treated females had ova in the ampulla and a vaginal plug after being caged with males but none became pregnant. Ovaries from inbred control or DES-treated females were grafted to the ovarian bursa of control or DES-treated ovariectomized hosts. DES-treated hosts, carrying control or DES-exposed ovaries, never became pregnant. Control females, with control ovaries or DES-exposed ovaries, became pregnant; pregnancy rate and litter size were similar for control mice regardless of whether they were supporting DES-exposed or control ovaries. Oocytes from ovaries exposed neonatally to DES can thus give rise to apparently normal offspring. The results also indicate DES-induced nonovarian disturbances, e.g. tubal and/or endometrial function, both of which are important for fertility. In the grafting experiments, a high mortality rate was found in inbred DES-exposed females caged with males. All deaths were associated with vaginal concrements (vaginal stones) and intestinal complications.
近交系和随机繁殖的NMRI小鼠在出生后第1 - 5天接受己烯雌酚（DES，每天5微克）或赋形剂（橄榄油）处理。8周龄时，对雌性小鼠用生理盐水或eCG和hCG处理以诱导排卵。未交配时，生理盐水处理的和DES处理的雌性小鼠输卵管壶腹部均未出现卵子。促性腺激素处理后，所有橄榄油处理的雌性小鼠输卵管壶腹部发现有卵子，约80%的DES处理的雌性小鼠也有卵子。两组排卵的卵子数量相似。20%接受促性腺激素处理的DES处理的雌性小鼠与雄性小鼠关在一起后，输卵管壶腹部有卵子且有阴道栓，但无一怀孕。将近交系对照或DES处理的雌性小鼠的卵巢移植到对照或DES处理的去卵巢宿主的卵巢囊。携带对照或DES暴露卵巢的DES处理的宿主从未怀孕。对照雌性小鼠，无论其支持DES暴露卵巢还是对照卵巢，都能怀孕；对照小鼠的怀孕率和产仔数相似。因此，新生期暴露于DES的卵巢中的卵母细胞能产生明显正常的后代。结果还表明DES诱导了非卵巢干扰，例如输卵管和/或子宫内膜功能，这两者对生育都很重要。在移植实验中，发现与雄性小鼠关在一起的近交系DES暴露雌性小鼠死亡率很高。所有死亡均与阴道结石和肠道并发症有关。
