Department of Immunology, University of Connecticut Health Center, Farmington, CT, USA.
Mucosal Immunol. 2012 Nov;5(6):691-701. doi: 10.1038/mi.2012.42. Epub 2012 Jun 20.
In a biphasic, ovalbumin (OVA)-induced murine asthma model where allergic airway disease is followed by resolution and the development of local inhalational tolerance (LIT), transforming growth factor (TGF)-β-expressing CD5(+) B cells were selectively expanded locally in hilar lymph nodes (HLN) of LIT mice. LIT HLN CD5(+) B cells, but not LIT HLN CD5(-) B cells, induced expression of Foxp3 in CD4(+)CD25(-) T cells in vitro. These CD5(+) regulatory B cells (Breg) and CD4(+)Foxp3(+) T cells demonstrated similar increases in expression of chemokine receptors (CXCR4 and CXCR5) and co-localized in HLN B cell zones of LIT mice. The adoptive transfer of LIT HLN CD5(+) B cells, but not LIT HLN CD5(-) B cells, increased the number of CD4(+)Foxp3(+) T cells in the lung and inhibited airway eosinophilia in this OVA model. Thus, Breg in HLNs of LIT mice reside in a CD5(+) TGF-β-producing subpopulation and co-localize with CD4(+)Foxp3(+) T cells.
在一个双相的卵清蛋白（OVA）诱导的小鼠哮喘模型中，过敏气道疾病随后得到解决，并发展为局部吸入性耐受（LIT），转化生长因子（TGF）-β表达的 CD5（+）B 细胞在 LIT 小鼠的肺门淋巴结（HLN）中被选择性地局部扩增。LIT HLN CD5（+）B 细胞，但不是 LIT HLN CD5（-）B 细胞，在体外诱导 CD4（+）CD25（-）T 细胞中 Foxp3 的表达。这些 CD5（+）调节性 B 细胞（Breg）和 CD4（+）Foxp3（+）T 细胞表现出趋化因子受体（CXCR4 和 CXCR5）表达的相似增加，并在 LIT 小鼠的 HLN B 细胞区共存。LIT HLN CD5（+）B 细胞的过继转移，但不是 LIT HLN CD5（-）B 细胞，增加了肺部 CD4（+）Foxp3（+）T 细胞的数量，并抑制了该 OVA 模型中的气道嗜酸性粒细胞增多。因此，LIT 小鼠 HLN 中的 Breg 存在于 CD5（+）TGF-β产生的亚群中，并与 CD4（+）Foxp3（+）T 细胞共存。
