Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, AL 35294-0005, USA.
Brain Behav Immun. 2010 Jan;24(1):153-9. doi: 10.1016/j.bbi.2009.09.011. Epub 2009 Sep 23.
We have reported previously that moderate intensity aerobic exercise training attenuates airway inflammation in a murine asthma model. Recent studies implicate regulatory T (Treg) cells in decreasing asthma-related airway inflammation; as such, the current study examined the effect of exercise on Treg cell function in a murine asthma model. Mice were sensitized with ovalbumin (OVA) prior to the start of exercise training at a moderate intensity 3x/week for 4weeks; exercise was performed as treadmill running (13.5m/min, 0% grade). Mice were OVA challenged repeatedly throughout the exercise protocol. At protocol completion, mice were analyzed for changes in the number and suppressive function of CD4(+)CD25(+)Foxp3(+) cells isolated from lungs, mediastinal lymph nodes, and spleens. Results show that exercise increased significantly the number of Foxp3(+) cells within the lungs and mediastinal lymph nodes, but not the spleens, of OVA-treated mice as compared with sedentary controls. Exercise also enhanced the suppression function of CD4(+)CD25(+)Foxp3(+) Treg cells derived from OVA-treated mice as compared with sedentary controls. Specifically, Treg cells from exercised, OVA-treated mice more effectively suppressed CD4(+)CD25(-) cell proliferation and Th2 cytokine production in vitro. Enhanced suppression was associated with increased protein levels of TGF-beta and lesser amounts of IL-10 and IL-17; however, blocking TGF-beta had no effect on suppressive functions. These data demonstrate that exercise-mediated increases in Treg cell function may play a role in the attenuation of airway inflammation. Further, these results indicate that moderate intensity aerobic exercise training may alter the Treg cell function within the asthmatic airway.
我们之前已经报道过，中等强度的有氧运动训练可以减轻哮喘小鼠模型中的气道炎症。最近的研究表明调节性 T（Treg）细胞可以减少与哮喘相关的气道炎症；因此，本研究检测了运动对哮喘小鼠模型中 Treg 细胞功能的影响。在开始每周 3 次、每次 4 周的中等强度运动训练之前，用卵清蛋白（OVA）对小鼠进行敏化；运动方式为跑步机跑步（13.5m/min，0%坡度）。在整个运动方案中，反复对小鼠进行 OVA 挑战。在方案完成时，分析了从肺部、纵隔淋巴结和脾脏中分离出的 CD4(+)CD25(+)Foxp3(+)细胞的数量和抑制功能的变化。结果表明，与安静对照组相比，运动显著增加了 OVA 处理小鼠肺部和纵隔淋巴结中 Foxp3(+)细胞的数量，但对脾脏中 Foxp3(+)细胞的数量没有影响。运动还增强了从 OVA 处理小鼠中分离出的 CD4(+)CD25(+)Foxp3(+)Treg 细胞的抑制功能，与安静对照组相比。具体来说，与安静对照组相比，来自运动处理、OVA 处理的小鼠的 Treg 细胞更有效地抑制了 CD4(+)CD25(-)细胞的增殖和 Th2 细胞因子的产生。增强的抑制作用与 TGF-β蛋白水平升高和 IL-10 和 IL-17 水平降低有关；然而，阻断 TGF-β对抑制功能没有影响。这些数据表明，运动介导的 Treg 细胞功能增加可能在减轻气道炎症中发挥作用。此外，这些结果表明，中等强度的有氧运动训练可能改变哮喘气道中的 Treg 细胞功能。
