Wang Xiao-Jun, Chen Shao-Hua, Zhang Zhao-Ji, Xiao Jun-Chao
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
Huan Jing Ke Xue. 2012 Apr;33(4):1306-12.
A horizontal subsurface flow constructed wetland was used to treat micro-pollution surface water and its effluent flowed into a landscape river as the water supplement. The abundance of the nitrous oxide reductase gene nosZ and 16S rDNA was investigated for the rhizosphere soil of the constructed wetland and the sediment of the landscape river by quantitive PCR. The diversity and similarity of denitrifiers were studied by using PCR-DGGE technique targeting the functional gene nosZ. The quantitive PCR results showed that the copy number of 16S rDNA and nosZ gene of the rhizosphere soil of the constructed wetland and the sediment of the landscape river were 1.91E + 07, 1.26E + 06 and 2.68E + 07, 8.37E + 05 copies x ng(-1) (by DNA), and 1.45E + 11, 9.31E + 09 and 5.31E + 11, 1.45E + 10 copies x g(-1) (by dry soil), respectively. Generally, the total bacterial count and denitrifiers in the sediment of the landscape river was higher than that of the constructed wetland, but the relative abundance of nosZ of the latter (3.8% - 10.1%) was significantly higher than that of the former (1.7% - 4.1%). There was low similarity between the two niches in cluster analysis. The predominant species of rhizosphere soil were mainly contained Rhodobacearales, Rhizobiales and Burkholderiales, although the predominant species of sediment were uncultured bacteria.
采用水平潜流人工湿地处理微污染地表水，其出水作为景观河的补水流入景观河。通过定量PCR研究了人工湿地根际土壤和景观河沉积物中一氧化二氮还原酶基因nosZ和16S rDNA的丰度。利用针对功能基因nosZ的PCR-DGGE技术研究了反硝化细菌的多样性和相似性。定量PCR结果表明，人工湿地根际土壤和景观河沉积物中16S rDNA和nosZ基因的拷贝数分别为1.91E + 07、1.26E + 06和2.68E + 07、8.37E + 05拷贝×ng(-1)(以DNA计)，以及1.45E + 11、9.31E + 09和5.31E + 11、1.45E + 10拷贝×g(-1)(以干土计)。总体而言，景观河沉积物中的细菌总数和反硝化细菌数量高于人工湿地，但人工湿地中nosZ的相对丰度(3.8% - 10.1%)显著高于景观河沉积物(1.7% - 4.1%)。聚类分析表明，两个生态位之间的相似性较低。根际土壤的优势种主要包括红螺菌目、根瘤菌目和伯克氏菌目，而沉积物的优势种为未培养细菌。
