Yan Pingmei, Qiao Hongping, Zhao Wenjing, Chen Yanfei, Shan Shuhua, Cao Aocheng
Wei Sheng Wu Xue Bao. 2015 Jan 4;55(1):73-9.
We studied the effect of methyl bromide fumigation on soil edaphic denitrification.
We adopted nosZ-PCR-RFLP (restriction fragment length polymorphism) method, nosZ-MPN-PCR (Most-Probable-Number- PCR) counting method and soil nitrate elimination rate method, to explore the effect of methyl bromide fumigation on community structure, quantity and activity of denitrifying bacteria in soil.
After methyl bromide fumigating soil for 100 d, soil denitrification did not change obviously (P > 0. 05). Margalef index, Shannon-wiener index and Evenness index had no significant difference (P > 0.05) in nosZ denitrifying bacterial communities between fumigated soil and the control. There were Rhodopsendomonas, Pseudomonas fluorescens, Herbacspirillum, uncultured bacterium partial in both of them. However, Azospirillum, Rhizobium melibei, Nitrosospira multiformis were exclusively found in the control, and Uncultured Azospirillum sp, Mesorhizobium sp were in fumigated one. Moreover, the number of denitrifying bacteria in the control resolved by nosZ-MPN-PCR (Most-Probable-Number-PCR) was 1.4 times higher than that of the fumigated one.
After 100 d fumigating soil, the composition of nosZ denitrifying microbial community and the population of denitrifying bacteria changed. Furthermore, there was no difference in denitrification between the fumigated soil and the control.
我们研究了溴甲烷熏蒸对土壤中土壤反硝化作用的影响。
我们采用nosZ-PCR-RFLP（限制性片段长度多态性）方法、nosZ-MPN-PCR（最大可能数-PCR）计数法和土壤硝酸盐消除率法，来探究溴甲烷熏蒸对土壤中反硝化细菌群落结构、数量及活性的影响。
溴甲烷熏蒸土壤100天后，土壤反硝化作用无明显变化（P>0.05）。熏蒸土壤与对照土壤中nosZ反硝化细菌群落的Margalef指数、Shannon-wiener指数和均匀度指数无显著差异（P>0.05）。两者中均存在红假单胞菌、荧光假单胞菌、草螺菌、未培养细菌部分。然而，固氮螺菌、苜蓿根瘤菌、多形亚硝化单胞菌仅在对照土壤中发现，未培养的固氮螺菌属、中生根瘤菌属在熏蒸土壤中发现。此外，通过nosZ-MPN-PCR（最大可能数-PCR）解析的对照土壤中反硝化细菌数量比熏蒸土壤中的高1.4倍。
熏蒸土壤100天后，nosZ反硝化微生物群落组成及反硝化细菌数量发生了变化。此外，熏蒸土壤与对照土壤之间的反硝化作用无差异。
