Key Laboratory of Yangtze Water Environment of Ministry of the State Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China; Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, 16521 Prague 6, Czech Republic.
Key Laboratory of Yangtze Water Environment of Ministry of the State Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China.
Bioresour Technol. 2014 Apr;157:341-5. doi: 10.1016/j.biortech.2014.01.137. Epub 2014 Feb 11.
The effect of Typha latifolia and its litter on density and abundance of three denitrifying genes (nirS, nirK and nosZ) were investigated in six laboratory-scale SSF CW microcosms. Results showed that the copy numbers of nirS, nirK and nosZ in wetland microcosms were ranged between 10(8)-10(9), 10(6)-10(7) and 10(7)-10(8) copies g(-1), respectively. The presence of T. latifolia encouraged the growth of nirK containing bacteria. Addition of cattail litter could greatly stimulate the growth of bacteria containing nirS and nosZ gene. Path analysis illustrated that the presence of plants and litters had no significant direct impact on denitrifying genes, while it affected the denitrifying genes via alteration of dissolved oxygen and carbon sources.
研究了香蒲及其凋落物对 6 个实验室规模 SSF CW 微宇宙中三种反硝化基因（nirS、nirK 和 nosZ）密度和丰度的影响。结果表明，湿地微宇宙中 nirS、nirK 和 nosZ 的拷贝数分别在 10(8)-10(9)、10(6)-10(7)和 10(7)-10(8)拷贝 g(-1)之间。香蒲的存在促进了含有 nirK 的细菌的生长。添加香蒲凋落物可极大地刺激含有 nirS 和 nosZ 基因的细菌的生长。路径分析表明，植物和凋落物的存在对反硝化基因没有显著的直接影响，而是通过改变溶解氧和碳源来影响反硝化基因。
