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m:Explorer：多项回归模型揭示了酵母休眠中长寿的正调控因子和负调控因子。

m:Explorer: multinomial regression models reveal positive and negative regulators of longevity in yeast quiescence.

机构信息

EMBL-European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge, UK.

出版信息

Genome Biol. 2012 Jun 21;13(6):R55. doi: 10.1186/gb-2012-13-6-r55.

DOI:10.1186/gb-2012-13-6-r55
PMID:22720667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3446321/
Abstract

We developed m:Explorer for identifying process-specific transcription factors (TFs) from multiple genome-wide sources, including transcriptome, DNA-binding and chromatin data. m:Explorer robustly outperforms similar techniques in finding cell cycle TFs in Saccharomyces cerevisiae. We predicted and experimentally tested regulators of quiescence (G0), a model of ageing, over a six-week time-course. We validated nine of top-12 predictions as novel G0 TFs, including Δmga2, Δcst6, Δbas1 with higher viability and G0-essential TFs Tup1, Swi3. Pathway analysis associates longevity to reduced growth, reprogrammed metabolism and cell wall remodeling. m:Explorer (http://biit.cs.ut.ee/mexplorer/) is instrumental in interrogating eukaryotic regulatory systems using heterogeneous data.

摘要

我们开发了 m:Explorer，用于从多个全基因组资源中识别特定于过程的转录因子（TFs），包括转录组、DNA 结合和染色质数据。m:Explorer 在识别酿酒酵母中的细胞周期 TF 方面，性能优于类似的技术。我们在六周的时间过程中，预测并实验测试了静止（G0）的调节剂，这是衰老的模型。我们验证了前 12 个预测中的九个为新型 G0 TF，包括 Δmga2、Δcst6、Δbas1，它们具有更高的存活率和 G0 必需的 TF Tup1、Swi3。通路分析将长寿与生长减缓、代谢重编程和细胞壁重塑联系起来。m:Explorer（http://biit.cs.ut.ee/mexplorer/）在使用异构数据探究真核调控系统方面具有重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/97e96fba57fb/gb-2012-13-6-r55-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/252eb06ae79a/gb-2012-13-6-r55-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/1f2f169495b3/gb-2012-13-6-r55-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/c6727e8d1133/gb-2012-13-6-r55-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/188a1d699524/gb-2012-13-6-r55-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/86a2a37efa42/gb-2012-13-6-r55-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/97e96fba57fb/gb-2012-13-6-r55-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/252eb06ae79a/gb-2012-13-6-r55-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/1f2f169495b3/gb-2012-13-6-r55-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/c6727e8d1133/gb-2012-13-6-r55-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/188a1d699524/gb-2012-13-6-r55-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/86a2a37efa42/gb-2012-13-6-r55-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3228/3446321/97e96fba57fb/gb-2012-13-6-r55-6.jpg

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