Tahmasebi A, Dizadji A, Farhoudi F, Allahyari H, Koohi-Habibi M
Acta Virol. 2012;56(2):139-43. doi: 10.4149/av_2012_02_139.
Cucumber mosaic virus (CMV) is one of the most important legume-infecting viruses, which is transmitted effectively by pea aphid Acyrthosiphon pisum (Harris) (Hem: Aphididae). Transmission efficiency of two CMV isolates (As and Kh from cowpea and bean hosts, resp.) by red and green color morphs of pea aphid were evaluated on bean plants. Triple-antibody sandwich ELISA (TAS-ELISA) using CMV-specific monoclonal antibodies revealed that both CMV isolates belonged to the serotype II. Bean plants inoculated by viruliferous aphids were assayed by double-antibody sandwich ELISA (DAS-ELISA) at 16 days post inoculation (dpi). The results showed that the transmission rate of CMV-As by the red morph was significantly higher than by the green morph, resulting in significantly higher transmission rate of the virus (As + Kh) by the red morph than by the green morph, with p≤ 0.1. Similarly, the efficiency of CMV transmission by the red morph of A. pisum was higher than the efficiency of transmission by the green morph. The higher transmission rate and efficiency of CMV by red pea aphid would be important in the epidemiology. Based on these results, we hypothesize that the transmission efficiency of CMV is affected more by the difference in transmission determinants of A. pisum color morphs than by the sequence of virus coat protein determinants.
Aphididae; Bromoviridae; color polymorphism; transmission efficiency.
黄瓜花叶病毒（CMV）是最重要的感染豆科植物的病毒之一，它可由豌豆蚜（Acyrthosiphon pisum (Harris)，半翅目：蚜科）有效传播。在菜豆植株上评估了豌豆蚜红色和绿色形态对两种CMV分离株（分别来自豇豆和菜豆寄主的As和Kh）的传播效率。使用CMV特异性单克隆抗体的三抗体夹心ELISA（TAS-ELISA）表明，两种CMV分离株均属于血清型II。在接种带毒蚜虫16天后，通过双抗体夹心ELISA（DAS-ELISA）对接种的菜豆植株进行检测。结果表明，红色形态豌豆蚜对CMV-As的传播率显著高于绿色形态，导致红色形态对病毒（As + Kh）的传播率显著高于绿色形态，p≤0.1。同样，豌豆蚜红色形态对CMV的传播效率高于绿色形态。红色豌豆蚜对CMV较高的传播率和效率在流行病学中具有重要意义。基于这些结果，我们推测CMV的传播效率受豌豆蚜颜色形态传播决定因素差异的影响大于病毒外壳蛋白决定因素的序列。
蚜科；雀麦花叶病毒科；颜色多态性；传播效率
