Department of Chemistry, Queen's University, Kingston, Ontario, Canada K7L 3N6.
Bioresour Technol. 2012 Aug;118:628-32. doi: 10.1016/j.biortech.2012.05.084. Epub 2012 May 29.
A switchable hydrophilicity solvent (SHS) was studied for its effectiveness at extracting lipids from freeze-dried samples of Botryococcus braunii microalgae. The SHS N,N-dimethylcyclohexylamine extracted up to 22 wt.% crude lipid relative to the freeze-dried cell weight. The solvent was removed from the extract with water saturated with carbon dioxide at atmospheric pressure and recovered from the water upon de-carbonation of the mixture. Liquid chromatography-mass spectrometry (LC-MS) showed that the extracted lipids contained high concentrations of long chain tri-, di- and mono-acylglycerols, no phospholipids, and only 4-8% of residual solvent. Unlike extractions with conventional organic solvents, this new method requires neither distillation nor the use of volatile, flammable or chlorinated organic solvents.
一种可切换亲水性溶剂（SHS）被研究用于从冷冻干燥的 Botryococcus braunii 微藻样品中提取脂质的效果。SHS N，N-二甲基环己胺相对于冷冻干燥的细胞重量提取了高达 22wt%的粗脂质。溶剂在大气压下用二氧化碳饱和水从提取物中除去，并在混合物脱碳化后从水中回收。液相色谱-质谱（LC-MS）表明，提取的脂质含有高浓度的长链三酰基、二酰基和单酰基甘油，不含磷脂，残留溶剂仅为 4-8%。与传统有机溶剂提取不同，这种新方法既不需要蒸馏，也不需要使用挥发性、易燃或含氯有机溶剂。
