Department of Chemistry, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Department of Civil Engineering, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Bioresour Technol. 2015 May;184:286-290. doi: 10.1016/j.biortech.2014.11.111. Epub 2014 Dec 8.
The use of CO2-expanded methanol (cxMeOH) and liquid carbon dioxide (lCO2) is proposed to extract lipids from Botryococcus braunii. When compressed CO2 dissolves in methanol, the solvent expands in volume, decreases in polarity and so increases in its selectivity for biodiesel desirable lipids. Solid phase extraction of the algal extract showed that the cxMeOH extracted 21 mg of biodiesel desirable lipids per mL of organic solvent compared to 3mg/mL using either neat methanol or chloroform/methanol mixture. The non-polar lCO2 showed a high affinity for non-polar lipids. Using lCO2, it is possible to extract up to 10% neutral lipids relative to the mass of dry algae. Unlike extractions using conventional solvents, these new methods require little to no volatile, flammable, or chlorinated organic solvents.
拟用 CO2 膨胀甲醇（cxMeOH）和液态二氧化碳（lCO2）从柏油球藻中提取脂质。当压缩 CO2 溶解在甲醇中时，溶剂体积膨胀，极性降低，因此对生物柴油所需脂质的选择性增加。藻类提取物的固相萃取表明，与使用纯甲醇或氯仿/甲醇混合物相比，每毫升有机溶剂 cxMeOH 可提取 21mg 生物柴油所需脂质，而每毫升有机溶剂则可提取 3mg/mL。非极性 lCO2 对非极性脂质具有高亲和力。使用 lCO2，可以从干燥藻类的质量中提取高达 10%的中性脂质。与使用传统溶剂的提取方法不同，这些新方法几乎不需要使用挥发性、易燃或含氯有机溶剂。
