Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, Zhejiang University, Hangzhou 310058, People's Republic of China.
Gene. 2012 Sep 1;505(2):254-8. doi: 10.1016/j.gene.2012.06.015. Epub 2012 Jun 18.
The accumulating evidence indicates that the viral structural proteins play critical roles in virus infection. However, the interaction between the viral structural protein and host cytoskeleton protein in virus infection remains to be addressed. In this study, the viral VP466 protein, one of the major structural proteins of shrimp white spot syndrome virus (WSSV), was characterized. The results showed that the suppression of VP466 gene expression led to the inhibition of WSSV infection in shrimp, indicating that the VP466 protein was required in virus invasion. It was found that the VP466 protein was interacted with the host cytoskeleton protein tropomyosin. As documented, the VP466-tropomyosin interaction facilitated the WSSV infection. Therefore our findings revealed a novel molecular mechanism in the virus invasion to its host, which would be helpful to better understand the molecular events in virus infection in invertebrate.
积累的证据表明，病毒结构蛋白在病毒感染中起着关键作用。然而，病毒结构蛋白与宿主细胞骨架蛋白在病毒感染中的相互作用仍有待解决。本研究对虾白斑综合征病毒（WSSV）的主要结构蛋白之一 VP466 蛋白进行了表征。结果表明，抑制 VP466 基因表达会抑制虾的 WSSV 感染，表明 VP466 蛋白是病毒入侵所必需的。研究发现，VP466 蛋白与宿主细胞骨架蛋白原肌球蛋白相互作用。有文献记载，VP466-原肌球蛋白的相互作用促进了 WSSV 的感染。因此，我们的研究结果揭示了病毒入侵宿主的一种新的分子机制，这将有助于更好地理解无脊椎动物病毒感染中的分子事件。
