Ha Yu-Mi, Soo-Jung Gong, Thi-Hoai Nguyen, Ra Chae Hun, Kim Ki-Hong, Nam Yoon-Kwon, Kim Sung-Koo
Department of Biotechnology and Bioengineering,Pukyong National University, Busan 608-737, Korea.
J Microbiol Biotechnol. 2008 May;18(5):964-7.
Two structural protein genes, VP19 and VP466, of white spot syndrome virus (WSSV) were cloned and expressed in Sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against WSSV for shrimps. The cumulative mortalities of the shrimps vaccinated by the injection of rVP19 and rVP466 at 15 days after the challenge with WSSV were 50.2% and 51.8%, respectively. For the vaccination by oral feeding of rVP19 and rVP466, the cumulative mortalities were 49.2% and 89.2%, respectively. These results show that protection against WSSV can be generated in the shrimp, using the viral structural protein as a protein vaccine.
利用杆状病毒表达系统，克隆了白斑综合征病毒（WSSV）的两个结构蛋白基因VP19和VP466，并在Sf21昆虫细胞中进行表达，以开发针对虾的注射和口服WSSV疫苗。在用WSSV攻击后15天，注射rVP19和rVP466疫苗的虾的累积死亡率分别为50.2%和51.8%。对于口服rVP19和rVP466疫苗，累积死亡率分别为49.2%和89.2%。这些结果表明，使用病毒结构蛋白作为蛋白疫苗可以在虾中产生针对WSSV的保护作用。
