Environmental Health & Industrial Safety Program, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Bull Environ Contam Toxicol. 2012 Aug;89(2):225-8. doi: 10.1007/s00128-012-0708-6. Epub 2012 Jun 22.
Glutathione-S-Transferase (GST) and metallothionein are important biomarker endpoints in studying the effect of Cd exposure. The purpose of this research was to study the correlation between hepatic GST and metallothionein with hepatic Cd in wild Fejervarya limnocharis exposed to environmental Cd. Results showed that frogs from contaminated sites had significantly higher hepatic metallothionein (3.58 mg/kg wet weight) and GST activity (0.259 μmol/min/mg total protein) than those from the reference site (2.36 mg/kg wet weight and 0.157 μmol/min/mg total protein respectively). There was a significantly positive correlation between hepatic Cd and GST activity (r = 0.802, p = 0.009) but not between hepatic Cd and metallothionein (r = 0.548, p = 0.139). The results concluded that while frogs from the contaminated site had higher GST and metallothionein, only GST showed significant positive correlation with hepatic Cd levels, indicating that hepatic GST activity may be used as a biomarker endpoint.
谷胱甘肽-S-转移酶 (GST) 和金属硫蛋白是研究镉暴露影响的重要生物标志物终点。本研究旨在研究暴露于环境镉的野生爪蟾肝脏 GST 和金属硫蛋白与肝脏镉之间的相关性。结果表明，污染地区的青蛙肝脏金属硫蛋白（3.58mg/kg 湿重）和 GST 活性（0.259μmol/min/mg 总蛋白）明显高于对照点（分别为 2.36mg/kg 湿重和 0.157μmol/min/mg 总蛋白）。肝脏镉与 GST 活性之间呈显著正相关（r=0.802，p=0.009），但与肝脏镉与金属硫蛋白之间无相关性（r=0.548，p=0.139）。研究结果表明，尽管污染地区的青蛙具有更高的 GST 和金属硫蛋白，但只有 GST 与肝脏镉水平呈显著正相关，表明肝脏 GST 活性可用作生物标志物终点。
