Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China; The College of Fisheries, Ocean University of China, Qingdao, PR China.
Aquat Toxicol. 2019 Sep;214:105255. doi: 10.1016/j.aquatox.2019.105255. Epub 2019 Jul 15.
The heavy metal cadmium readily accumulates in organisms, causing damage. In this study, juvenile marine shrimp Marsupenaeus japonicus were exposed to cadmium (Cd; 5, 50 and 500 μg L). Cd accumulation and antioxidant-related indices were determined, and damage to biomolecules was assessed, after 24, 48 and 96 h. Cd bioaccumulation in M. japonicus increased with exposure time and concentration, which reached the highest value at 96 h. The data showed that 5, 50 and 500 μg L Cd increased glutathione (GSH) content and the activities of superoxide dismutase (SOD) and glutathione S-transferase (GST) in a Cd-dose-dependent manner, but 5 and 50 μg L Cd had no effect on caspase-3 activity. The expression levels of SOD, GST, heat shock protein 70 (HSP70), metallothionein (MT), p53 and caspase-3 genes were rapidly increased after 50 and 500 μg L Cd exposure, and remained at a significantly higher level than in the control after 96 h of exposure. After exposure to 5, 50 and 500 μg L Cd, F-value (the ratio between double-stranded DNA and total DNA) remained high at 24 h, however, as the exposure time increased, the F-value decreased in a dose-dependent manner. An increase in malondialdehyde content was also observed following exposure to 50 and 500 μg L Cd. Our data suggest that Cd induces oxidative stress, molecular damage and apoptosis in juvenile M. japonicus in a concentration- and time-dependent manner.
重金属镉容易在生物体内积累，造成损害。本研究采用日本沼虾幼虾作为实验对象，将其暴露于镉（Cd；5、50 和 500μg/L）中，24、48 和 96 h 后测定 Cd 积累和抗氧化相关指标，并评估生物分子损伤情况。Cd 在 M. japonicus 体内的生物积累随暴露时间和浓度的增加而增加，在 96 h 时达到最高值。结果表明，5、50 和 500μg/L Cd 以剂量依赖的方式增加了谷胱甘肽（GSH）含量和超氧化物歧化酶（SOD）、谷胱甘肽 S-转移酶（GST）的活性，但 5 和 50μg/L Cd 对 caspase-3 活性没有影响。SOD、GST、热休克蛋白 70（HSP70）、金属硫蛋白（MT）、p53 和 caspase-3 基因的表达水平在暴露于 50 和 500μg/L Cd 后迅速增加，并且在暴露 96 h 后仍保持在显著高于对照的水平。暴露于 5、50 和 500μg/L Cd 后，F 值（双链 DNA 与总 DNA 的比值）在 24 h 时保持较高水平，但随着暴露时间的延长，F 值呈剂量依赖性下降。暴露于 50 和 500μg/L Cd 还导致丙二醛含量增加。本研究数据表明，Cd 以浓度和时间依赖的方式诱导日本沼虾幼虾产生氧化应激、分子损伤和细胞凋亡。
