Chen Y
Union Hospital, Tongji Medical College, Wuhan.
Zhonghua Zhong Liu Za Zhi. 1990 Jul;12(4):268-70.
By scanning and transmission electron microscopy, leukemic cells were observed after CFU-Mix culture. Even though granulocytic growth factor, erythropoietin and lymphocytic growth factor were added in vitro, acute leukemic cells still showed defective differentiation and maturation. These were characterized by abnormal colony which consisted of smooth cells, bizarre shape, nuclear-cytoplasmic asynchrony in development, and appearance of nuclear bleb. However, chronic myelogenous leukemic cells were more mature than the acute ones, manifesting in normal colony with finger-like projections and ruffled membrane. Macrophages and eosinophils could be observed. It is suggested that there is a difference in differentiation between acute and chronic leukemic cells.
通过扫描电子显微镜和透射电子显微镜观察，在CFU-Mix培养后观察到白血病细胞。即使在体外添加粒细胞生长因子、促红细胞生成素和淋巴细胞生长因子，急性白血病细胞仍表现出分化和成熟缺陷。其特征为异常集落，由光滑细胞组成，形态怪异，发育过程中核质不同步，出现核泡。然而，慢性粒细胞白血病细胞比急性白血病细胞更成熟，表现为具有指状突起和皱襞膜的正常集落。可观察到巨噬细胞和嗜酸性粒细胞。提示急性和慢性白血病细胞在分化方面存在差异。
