Chen Y, Wang B M, Li C Y, Yu D J
Chin Med J (Engl). 1989 Jun;102(6):425-30.
By limiting dilution assay, CFU-Mix of 47 cases of untreated leukemia, myelodysplastic syndrome and 30 cases of normal adults were studied. It is suggested that most cases of acute nonlymphocytic leukemia, like chronic myelogenous leukemia (CML) and myelodysplastic syndrome should have defect in pluripotent hemopoietic stem cell. The CFU-Mix of acute myelogenous leukemia (AML) and CML were obviously lower than that of normal adults (p less than 0.001). By light microscopy, scanning and transmission electron microscopy, it was confirmed that the CFU-Mix colonies, of leukemic patients were chiefly composed of leukemic cells. Their incorporating rate of 3H-TdR was high in the early stage of culture, but that of 55 + 59Fe was low in the whole course. This phenomenon was quite different from that of the normal adults in that the CFU-Mix of the patients showed distinctive proliferation and differentiation into the leukemic progenitor cells.
采用有限稀释法，对47例未经治疗的白血病、骨髓增生异常综合征患者及30例正常成年人的混合集落形成单位（CFU-Mix）进行了研究。结果提示，大多数急性非淋巴细胞白血病病例，如同慢性粒细胞白血病（CML）和骨髓增生异常综合征一样，多能造血干细胞存在缺陷。急性髓系白血病（AML）和CML的CFU-Mix明显低于正常成年人（p＜0.001）。通过光学显微镜、扫描和透射电子显微镜观察证实，白血病患者的CFU-Mix集落主要由白血病细胞组成。其在培养早期3H-TdR掺入率高，但在整个过程中55+59Fe掺入率低。这种现象与正常成年人截然不同，患者的CFU-Mix表现出独特的向白血病祖细胞增殖和分化的特性。
