Chen Y, Wang B M, Li C Y, Yu D J, Ruan Y B
Institute of Hematology, Tonji Medical University, Wuhan.
J Tongji Med Univ. 1990;10(1):27-30. doi: 10.1007/BF02909118.
By use of transmission electron microscope we demonstrated that the limiting dilution assay is an important method for investigating differentiation of hemopoietic cells in vitro. If various growth factors were added into this culture system, the stem cells of normal adults could differentiate into erythrocyte, granulocyte, megakaryocyte and macrophage, while leukemic progenitor cells could not. The colony forming cell in leukemia still had the nature of leukemic cell.
通过透射电子显微镜，我们证明有限稀释法是体外研究造血细胞分化的重要方法。如果在该培养系统中添加各种生长因子，正常成年人的干细胞可分化为红细胞、粒细胞、巨核细胞和巨噬细胞，而白血病祖细胞则不能。白血病中的集落形成细胞仍具有白血病细胞的特性。
