Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
PLoS One. 2012;7(6):e38532. doi: 10.1371/journal.pone.0038532. Epub 2012 Jun 18.
Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through isobaric tags for relative and absolute quantification (iTRAQ). Undifferentiated hESCs and hESCs in different stages of spontaneous differentiation by embryoid body (EB) formation were analyzed. Using the iTRAQ approach, we identified 156 differentially expressed proteins involved in cell proliferation, apoptosis, transcription, translation, mRNA processing, and protein synthesis. Proteins involved in nucleic acid binding, protein synthesis, and integrin signaling were downregulated during differentiation, whereas cytoskeleton proteins were upregulated. The present findings added insight to our understanding of the mechanisms involved in hESC proliferation and differentiation.
通过分析基因表达来定义涉及人胚胎干细胞（hESC）增殖和分化的分子机制和途径，进一步揭示了 hESC 的自我更新和多能性特征。通过等压标记相对和绝对定量（iTRAQ）发现与 hESC 相关的蛋白质。分析了未分化的 hESC 和通过胚状体（EB）形成处于不同自发分化阶段的 hESC。使用 iTRAQ 方法，我们鉴定出 156 种参与细胞增殖、凋亡、转录、翻译、mRNA 处理和蛋白质合成的差异表达蛋白。在分化过程中，涉及核酸结合、蛋白质合成和整合素信号的蛋白质下调，而细胞骨架蛋白上调。本研究结果加深了我们对 hESC 增殖和分化相关机制的理解。
