Porcelli G, Di Jorio M, Ranieri M, Ranalli L, D'Acquarica L
Adv Exp Med Biol. 1979;120A:143-5. doi: 10.1007/978-1-4757-0926-1_14.
A method was developed to measure kininase activity in human urine. The method consists of dialysis of human centrifuged urine sample against phosphate buffer and partial fractionation of A-50 Sephadex column. The enzymatic property of urinary kininase, which destroys bradykinin when incubated, is estimated from its effect on a definite amount of bradykinin, using rat uterus.
已开发出一种测量人尿中激肽酶活性的方法。该方法包括将人离心尿液样本用磷酸盐缓冲液进行透析，以及通过A - 50葡聚糖凝胶柱进行部分分级分离。利用大鼠子宫，根据尿激肽酶对一定量缓激肽的作用来评估其酶学性质，该酶在孵育时会破坏缓激肽。
