Department of Food Science, Aarhus University, Tjele 8830, Denmark.
BMC Gastroenterol. 2012 Jun 24;12:76. doi: 10.1186/1471-230X-12-76.
Accurate diagnostic and monitoring tools for ulcerative colitis (UC) are missing. Our aim was to describe the proteomic profile of UC and search for markers associated with disease exacerbation. Therefore, we aimed to characterize specific proteins associated with inflamed colon mucosa from patients with acute UC using mass spectrometry-based proteomic analysis.
Biopsies were sampled from rectum, sigmoid colon and left colonic flexure from twenty patients with active proctosigmoiditis and from four healthy controls for proteomics and histology. Proteomic profiles of whole colonic biopsies were characterized using 2D-gel electrophoresis, and peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for identification of differently expressed protein spots.
A total of 597 spots were annotated by image analysis and 222 of these had a statistically different protein level between inflamed and non-inflamed tissue in the patient group. Principal component analysis clearly grouped non-inflamed samples separately from the inflamed samples indicating that the proteomic signature of colon mucosa with acute UC is strong. Totally, 43 individual protein spots were identified, including proteins involved in energy metabolism (triosephosphate isomerase, glycerol-3-phosphate-dehydrogenase, alpha enolase and L-lactate dehydrogenase B-chain) and in oxidative stress (superoxide dismutase, thioredoxins and selenium binding protein).
A distinct proteomic profile of inflamed tissue in UC patients was found. Specific proteins involved in energy metabolism and oxidative stress were identified as potential candidate markers for UC.
溃疡性结肠炎（UC）缺乏准确的诊断和监测工具。我们的目的是描述 UC 的蛋白质组特征，并寻找与疾病恶化相关的标志物。因此，我们旨在使用基于质谱的蛋白质组分析来描述来自急性 UC 患者的炎症结肠黏膜相关的特定蛋白质。
从二十名患有活动期直肠乙状结肠炎和四名健康对照者的直肠、乙状结肠和左结肠弯曲处采集活检组织，用于蛋白质组学和组织学研究。使用 2D 凝胶电泳对整个结肠活检组织的蛋白质组特征进行描述，并应用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的肽质量指纹图谱进行鉴定差异表达的蛋白质斑点。
通过图像分析注释了 597 个斑点，其中 222 个斑点在患者组中炎症和非炎症组织之间的蛋白质水平存在统计学差异。主成分分析清楚地将非炎症样本与炎症样本分开，表明急性 UC 结肠黏膜的蛋白质组特征很强。总共鉴定了 43 个单独的蛋白质斑点，包括参与能量代谢（磷酸丙糖异构酶、甘油-3-磷酸脱氢酶、α烯醇酶和 L-乳酸脱氢酶 B 链）和氧化应激（超氧化物歧化酶、硫氧还蛋白和硒结合蛋白）的蛋白质。
发现 UC 患者炎症组织存在独特的蛋白质组特征。鉴定出参与能量代谢和氧化应激的特定蛋白质可能是 UC 的潜在候选标志物。
