Department of Ophthalmology, University of California San Francisco, San Francisco, California 94143, USA.
J Cataract Refract Surg. 2012 Jul;38(7):1266-70. doi: 10.1016/j.jcrs.2012.01.034.
To develop a rabbit model for continuous curvilinear capsulorhexis (CCC) instruction.
University of California San Francisco, San Francisco, California, USA.
Experimental study.
Isolated rabbit lenses were immersed in 2% to 8% paraformaldehyde (PFA) fixative from 15 minutes to 6 hours. Rabbit eyes were treated by substituting aqueous with 2% to 4% PFA for 30 minutes to 6 hours, followed by washes with a balanced salt solution. Treated lenses and eyes were held in purpose-designed holders using vacuum. A panel of 6 cataract surgeons with 5 to 15 years of experience performed CCC on treated lenses and eyes and responded to a questionnaire regarding the utility of these models for resident teaching using a 5-item Likert scale.
The expert panel found that rabbit lenses treated with increasing amounts of fixative simulated CCC on human lens capsules from the third to the seventh decade of life. The panel also found fixative-treated rabbit eyes to simulate some of the experience of CCC within the human anterior chamber but noted a shallower anterior chamber depth, variation in pupil size, and corneal clouding under some treatment conditions.
Experienced cataract surgeons who performed CCC on these rabbit models strongly agreed that isolated rabbit lenses treated with fixative provide a realistic simulation of CCC in human patients and that both models were useful tools for capsulorhexis instruction. Results indicate that rabbit lenses treated with 8% PFA for 15 minutes is a model with good fidelity for CCC training.
No author has a financial or proprietary interest in any material or method mentioned.
开发一种用于连续曲线撕囊术（CCC）教学的兔模型。
美国加利福尼亚大学旧金山分校，旧金山，加利福尼亚州。
实验研究。
将离体兔晶状体浸泡在 2%至 8%多聚甲醛（PFA）固定液中 15 分钟至 6 小时。用 2%至 4% PFA 替代水，将兔眼处理 30 分钟至 6 小时，然后用平衡盐溶液冲洗。用特制的真空夹具固定处理后的晶状体和眼睛。由 6 位具有 5 至 15 年经验的白内障外科医生组成的专家组对处理后的晶状体和眼睛进行 CCC，并使用 5 项李克特量表对这些模型在住院医师教学中的应用进行了问卷调查。
专家组发现，用固定剂处理的兔晶状体模拟了人类晶状体囊从第三到第七个十年的 CCC。专家组还发现，固定剂处理的兔眼在人类前房内模拟了一些 CCC 的经验，但注意到在前房深度较浅、瞳孔大小变化和在某些处理条件下角膜混浊等问题。
进行 CCC 的经验丰富的白内障外科医生强烈认为，用固定剂处理的离体兔晶状体提供了人类患者 CCC 的真实模拟，并且这两种模型都是囊膜撕囊术教学的有用工具。结果表明，用 8% PFA 处理 15 分钟的兔晶状体是 CCC 训练的一种具有良好逼真度的模型。
没有作者在任何材料或方法上有财务或所有权利益。
