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两例西班牙罕见种隐孢子虫属( Cryptosporidium ubiquitum 和 Cryptosporidium felis )引起的动物源隐孢子虫病。

Two cases of zoonotic cryptosporidiosis in Spain by the unusual species Cryptosporidium ubiquitum and Cryptosporidium felis.

机构信息

Area of Parasitology, Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza, Spain.

出版信息

Enferm Infecc Microbiol Clin. 2012 Nov;30(9):549-51. doi: 10.1016/j.eimc.2012.04.011. Epub 2012 Jun 23.

DOI:10.1016/j.eimc.2012.04.011
PMID:22728073
Abstract

INTRODUCTION

Two cases of infection by zoonotic transmission of unusual species of Cryptosporidium were detected in 2010-2011 in Spain (León and Zaragoza).

MATERIALS AND METHODS

Cryptosporidium spp. was detected by microscopic examination of modified Ziehl-Neelsen stained fecal smears. PCR-RFLP of the SSUrDNA gene and sequencing of the amplified fragment confirmed the species.

RESULTS

C. ubiquitum and C. felis were identified in samples from an immunocompetent child and from a HIV-positive adult, respectively.

CONCLUSIONS

This is the first report of human infection by C. ubiquitum (cervine) and autochthonous C. felis, identified in Spain.

摘要

简介

2010-2011 年在西班牙(莱昂和萨拉戈萨)检测到两例由罕见种属隐孢子虫引起的动物源性传染病。

材料与方法

通过改良的齐尔-尼尔森染色粪便涂片的显微镜检查来检测隐孢子虫属。SSUrDNA 基因的 PCR-RFLP 和扩增片段的测序确认了物种。

结果

在一名免疫功能正常的儿童和一名 HIV 阳性的成人的样本中分别鉴定出 C. ubiquitum 和 C. felis。

结论

这是在西班牙首次报告人感染 C. ubiquitum(鹿源)和本土 C. felis。

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