Baz-González Edgar, Martín-Carrillo Natalia, García-Livia Katherine, Foronda Pilar
Department Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, 38200 San Cristóbal de La Laguna, Tenerife, Canary Islands, Spain.
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, 38200 San Cristóbal de La Laguna, Tenerife, Canary Islands, Spain.
Vet Sci. 2022 Feb 18;9(2):91. doi: 10.3390/vetsci9020091.
is a zoonotic parasite responsible for cryptosporidiosis cases and outbreaks in both humans and rabbits. Since there are no molecular spp. infection data in rabbits () from Spain, our aim was to gather information about this parasite in wild European rabbits from Tenerife, Canary Islands (Spain). A total of 100 faecal samples were collected from rabbits from eight municipalities of Tenerife. Microscopic analysis showed that 4.0% of the samples presented structures compatible with oocyst. A nested polymerase chain reaction (PCR) targeting ribosomal RNA (rRNA) gene fragments was carried out, and sequencing confirmed the identity of in one sample (1.0%). The sample was successfully subtyped using nested PCR analysis of the 60-kDa glycoprotein () gene as the subtype VbA26R3. This study confirms the presence of in wild rabbits from Tenerife, providing new information on the occurrence of this zoonotic parasite. Further studies are required to better understand the epidemiology of spp. in wild rabbits in Spain and their possible public health repercussions.
是一种人畜共患寄生虫,可导致人类和兔子感染隐孢子虫病并引发疫情。由于西班牙没有关于兔子()感染该寄生虫的分子数据,我们的目标是收集来自加那利群岛特内里费岛(西班牙)野生欧洲兔体内这种寄生虫的信息。从特内里费岛八个市镇的兔子身上共采集了100份粪便样本。显微镜分析显示,4.0%的样本呈现出与卵囊相符的结构。对核糖体RNA(rRNA)基因片段进行了巢式聚合酶链反应(PCR),测序确认一个样本(1.0%)中存在该寄生虫。通过对60 kDa糖蛋白()基因进行巢式PCR分析,成功对该样本进行了亚型鉴定,为VbA26R3亚型。本研究证实了特内里费岛野生兔体内存在该寄生虫,为这种人畜共患寄生虫的发生情况提供了新信息。需要进一步研究以更好地了解西班牙野生兔体内该寄生虫的流行病学及其可能对公共卫生产生的影响。