Stenger Brianna L S, Horčičková Michaela, Clark Mark E, Kváč Martin, Čondlová Šárka, Khan Eakalak, Widmer Giovanni, Xiao Lihua, Giddings Catherine W, Pennil Christopher, Stanko Michal, Sak Bohumil, McEvoy John M
Department of Microbiological Sciences,North Dakota State University,Fargo, ND,USA.
Institute of Parasitology, Biology Centre of Czech Academy of Sciences,České Budějovice,Czech Republic.
Parasitology. 2018 Mar;145(3):326-334. doi: 10.1017/S0031182017001524. Epub 2017 Sep 5.
We undertook a study on Cryptosporidium spp. in wild cricetid rodents. Fecal samples were collected from meadow voles (Microtus pennsylvanicus), southern red-backed voles (Myodes gapperi), woodland voles (Microtus pinetorum), muskrats (Ondatra zibethicus) and Peromyscus spp. mice in North America, and from bank voles (Myodes glareolus) and common voles (Microtus arvalis) in Europe. Isolates were characterized by sequence and phylogenetic analyses of the small subunit ribosomal RNA (SSU) and actin genes. Overall, 33·2% (362/1089) of cricetids tested positive for Cryptosporidium, with a greater prevalence in cricetids from North America (50·7%; 302/596) than Europe (12·1%; 60/493). Principal Coordinate analysis separated SSU sequences into three major groups (G1-G3), each represented by sequences from North American and European cricetids. A maximum likelihood tree of SSU sequences had low bootstrap support and showed G1 to be more heterogeneous than G2 or G3. Actin and concatenated actin-SSU trees, which were better resolved and had higher bootstrap support than the SSU phylogeny, showed that closely related cricetid hosts in Europe and North America are infected with closely related Cryptosporidium genotypes. Cricetids were not major reservoirs of human pathogenic Cryptosporidium spp.
我们对野生仓鼠科啮齿动物中的隐孢子虫属进行了一项研究。粪便样本采集自北美洲的草甸田鼠（Microtus pennsylvanicus）、南部红背田鼠（Myodes gapperi）、林地田鼠（Microtus pinetorum）、麝鼠（Ondatra zibethicus）和白足鼠属小鼠，以及欧洲的小林姬鼠（Myodes glareolus）和普通田鼠（Microtus arvalis）。通过对小亚基核糖体RNA（SSU）和肌动蛋白基因进行序列和系统发育分析来鉴定分离株。总体而言，33.2%（362/1089）的仓鼠科动物隐孢子虫检测呈阳性，北美洲仓鼠科动物的患病率（50.7%；302/596）高于欧洲（12.1%；60/493）。主坐标分析将SSU序列分为三个主要组（G1 - G3），每组均由来自北美洲和欧洲仓鼠科动物的序列代表。SSU序列的最大似然树的自展支持率较低，且显示G1比G2或G3更具异质性。肌动蛋白树和串联的肌动蛋白 - SSU树比SSU系统发育树分辨率更高且自展支持率更高，表明欧洲和北美洲亲缘关系相近的仓鼠科宿主感染了亲缘关系相近的隐孢子虫基因型。仓鼠科动物并非人类致病性隐孢子虫属的主要储存宿主。
