Curso de Naturologia Aplicada, Unidade de Articulação da Saúde, Universidade do Sul de Santa Catarina, Pedra Branca, Palhoça, SC, Brazil.
J Ethnopharmacol. 2012 Aug 30;143(1):170-8. doi: 10.1016/j.jep.2012.06.020. Epub 2012 Jun 21.
Pterodon pubescens Benth is a medicinal plant commonly used for therapeutic purposes in folk medicine for rheumatic diseases' treatment. In the present work we analyzed the chemical composition of the oleaginous extract of P. pubescens Benth (OEPp) and extended the antinociceptive effect of OEPp evaluating its role on animal models of acute and chronic pain.
The antinociceptive and antiedematogenic effects of OEPp (3-100mg/kg, i.g.) were evaluated in the formalin test; mechanical allodynia in the postoperative pain and complex regional pain syndrome type-I (CRPS-I) animal models; and thermal hyperalgesia was induced by plantar incision. Finally, we performed a phytochemical analysis of OEPp.
The chemical composition of OEPp was analyzed by mass spectrometry (GC/MS) and eight sesquiterpene compounds were identified, i.e. three major sesquiterpene (E-cariofilene, γ-muurolene, biciclogermacrene), and nine vouacapane diterpenes, four of which showed in major concentration (6α-acetoxyvouacapane, 6α,7β-dimetoxivouacapan-17-ene, 6α-acetoxy,7β-hidroxyvouacapane, 6α,7β-diacetoxycouacapane). Furthermore, the results of the present study demonstrate, for the first time, that the OEPp reduced mechanical allodynia in the postoperative pain and CRPS-I animal models. OEPp also increased the paw withdrawal latency in hot- and cold-plate tests in the postoperative pain model. In addition, the present work confirms and extends previous data from literature showing that systemic administration of OEPp caused significant inhibition against both phases of pain response to formalin intraplantar injection and edema formation.
Together, present and previous findings show that OEPp given intra-gastrically caused significant inhibition against both phases of formalin intraplantar injection and effectively inhibited mechanical and thermal hyperalgesia in the postoperative pain and CRPS-I animal models.
毛瓣金花小檗（Pterodon pubescens Benth）是一种药用植物，常用于民间医学治疗风湿性疾病。在本工作中，我们分析了毛瓣金花小檗油性提取物（OEPp）的化学成分，并通过评估其在急性和慢性疼痛动物模型中的作用，扩展了 OEPp 的镇痛作用。
在福尔马林试验中评估了 OEPp（3-100mg/kg，ig）的镇痛和抗水肿作用；在术后疼痛和复杂性区域疼痛综合征 I 型（CRPS-I）动物模型中评估了机械性痛觉过敏；通过足底切口诱导热痛觉过敏。最后，我们对 OEPp 进行了植物化学分析。
通过质谱（GC/MS）分析了 OEPp 的化学成分，鉴定出八种倍半萜化合物，即三种主要倍半萜（E-石竹烯、γ-榄香烯、双环金缕梅烯）和九种缬草烷二萜，其中四种以主要浓度存在（6α-乙酰氧基缬草烷、6α，7β-二甲氧基缬草烷-17-烯、6α-乙酰氧基、7β-羟基缬草烷、6α，7β-二乙酰氧基考卡烷）。此外，本研究的结果首次表明，OEPp 减轻了术后疼痛和 CRPS-I 动物模型中的机械性痛觉过敏。OEPp 还增加了术后疼痛模型中热板和冷板试验中的足底回缩潜伏期。此外，本工作证实并扩展了文献中的先前数据，表明 OEPp 全身给药对福尔马林足底注射引起的疼痛反应的两个阶段以及水肿形成均有显著抑制作用。
综上所述，目前和以前的研究结果表明，OEPp 经胃内给药可显著抑制福尔马林足底注射引起的疼痛反应的两个阶段，并有效抑制术后疼痛和 CRPS-I 动物模型中的机械性和热痛觉过敏。
