Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão 121, Salvador, Bahia, Brazil.
J Ethnopharmacol. 2011 Jul 14;136(3):518-24. doi: 10.1016/j.jep.2010.05.065. Epub 2010 Jun 8.
Adiantum, one of the most widely distributed genera of the family Pteridaceae, is employed in folk medicine worldwide. Adiantum latifolium Lam. has been used in Latin American traditional medicine as anxiolytic, analgesic and antiinflammatory. The present study investigates the antinociceptive and antiinflammatory properties of the methanolic extract of Adiantum latifolium (MEA) in animal models of pain and inflammation to confirm its medicinal use.
The antinociceptive and antiinflammatory activities of MEA were evaluated using the writhing, formalin, and tail-flick tests, carrageenan-induced paw edema and arachidonic acid-induced ear edema. Mice motor performance was evaluated in the rota rod test and the acute toxicity evaluated over 14 days.
Intraperitoneal (1-100mg/kg) or oral (100-400mg/kg) administration of MEA produced a dose-related inhibition of acetic acid-induced writhing in mouse. Furthermore, treatment with MEA (100mg/kg) inhibited both the early and late phases of formalin-induced hypernociception. In contrast, MEA (100mg/kg/IP) did not prevent the thermal nociception in the tail-flick test. In addition, MEA (100 and 200mg/kg/IP) inhibited important events related to the inflammatory response induced by carrageenan or arachidonic acid, namely local edema and increase in tissue interleukin-1β levels. MEA (300mg/kg/IP)-treated mice did not show any motor performance alterations. Over the study period of 14 days, there were no deaths or toxic signs recorded in the group of mice given 1000mg/kg of MEA.
The results demonstrate that Adiantum latifolium has antinociceptive and antiinflammatory activities, acting through the inhibition of IL-1β production.
凤尾蕨科分布最广的属之一铁角蕨属，在世界各地的民间医学中被广泛应用。凤尾蕨（Adiantum latifolium Lam.）在拉丁美洲传统医学中被用作抗焦虑、镇痛和抗炎药。本研究旨在通过动物模型疼痛和炎症模型，研究凤尾蕨甲醇提取物（MEA）的镇痛和抗炎特性，以确认其药用价值。
采用扭体、福尔马林和甩尾试验、角叉菜胶诱导的爪水肿和花生四烯酸诱导的耳水肿评价 MEA 的镇痛和抗炎活性。通过转棒试验评价小鼠的运动性能，并在 14 天内评估急性毒性。
腹腔内（1-100mg/kg）或口服（100-400mg/kg）给予 MEA 可剂量依赖性抑制小鼠醋酸诱导的扭体反应。此外，MEA（100mg/kg）治疗可抑制福尔马林诱导的痛觉过敏的早期和晚期阶段。相比之下，MEA（100mg/kg/IP）不能防止甩尾试验中的热痛觉。此外，MEA（100 和 200mg/kg/IP）抑制了角叉菜胶或花生四烯酸诱导的炎症反应相关的重要事件，即局部水肿和组织白细胞介素-1β水平升高。MEA（300mg/kg/IP）治疗的小鼠未显示出任何运动性能改变。在为期 14 天的研究期间，给予 1000mg/kg MEA 的小鼠组未出现死亡或毒性迹象。
结果表明，凤尾蕨具有镇痛和抗炎活性，通过抑制 IL-1β 的产生发挥作用。
