Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., bl. 103A, BG-1113 Sofia, Bulgaria.
Int J Pharm. 2012 Oct 15;436(1-2):10-24. doi: 10.1016/j.ijpharm.2012.06.035. Epub 2012 Jun 21.
Nanofibrous polylactide-based materials loaded with a natural polyphenolic compound gossypol (GOS) with antitumor properties were prepared by electrospinning. The nanofibrous materials were coated with a thin film of crosslinked quaternized chitosan (QCh). GOS incorporated in the nanofibrous mats was in the amorphous state. GOS release was diffusion-controlled and its in vitro release profiles depended on the mat composition. The nanofibrous materials exhibited high cytotoxicity towards HeLa tumor cells. Interestingly, it was particularly pronounced in the case of fibrous materials, which contain both QCh and GOS. The observed strong antiproliferative effect of the nanofibrous mats was mainly due to induction of cell apoptosis.
采用静电纺丝法制备了载有具有抗肿瘤特性的天然多酚化合物棉酚（GOS）的纳米纤维聚乳酸基材料。将纳米纤维材料涂覆有交联季铵化壳聚糖（QCh）的薄膜。纳米纤维垫中掺入的 GOS 处于无定形状态。GOS 的释放是扩散控制的，其体外释放曲线取决于垫子的组成。纳米纤维材料对 HeLa 肿瘤细胞表现出高细胞毒性。有趣的是，对于同时含有 QCh 和 GOS 的纤维材料，这种现象尤为明显。观察到纳米纤维垫具有很强的抗增殖作用，主要是由于诱导细胞凋亡。
