Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St. bl. 103A, BG-1113 Sofia, Bulgaria.
Biomacromolecules. 2010 Jun 14;11(6):1633-45. doi: 10.1021/bm100285n.
Nanofibrous materials containing the antitumor drug doxorubicin hydrochloride (DOX) were easily prepared using a one-step method by electrospinning of DOX/poly(L-lactide-co-D,L-lactide) (coPLA) and DOX/quaternized chitosan (QCh)/coPLA solutions. The pristine and DOX-containing mats were characterized by ATR-FTIR and X-ray photoelectron spectroscopy (XPS). The release rate of DOX from the prepared fibers increased with the increase in DOX content. The DOX release process was diffusion-controlled. MTT cell viability studies revealed that incorporation of DOX and QCh in the nanofibrous mats led to a significant reduction in the HeLa cells viability. It was found, that the antitumor efficacy of the DOX-containing mats at 6 h was higher than that of the free DOX. SEM, TEM, and fluorescence microscopic observations confirmed that the antitumor effect of QCh-based and DOX-containing fibrous mats was mainly due to induction of apoptosis in the HeLa cells.
载盐酸多柔比星（DOX）的纳米纤维材料可通过静电纺丝一步法轻易制备，该方法使用 DOX/聚（L-丙交酯-co-D,L-丙交酯）（coPLA）和 DOX/季铵化壳聚糖（QCh）/coPLA 溶液。原纤维和含 DOX 的垫子通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）和 X 射线光电子能谱（XPS）进行了表征。从制备的纤维中释放 DOX 的速率随 DOX 含量的增加而增加。DOX 的释放过程为扩散控制。MTT 细胞活力研究表明，将 DOX 和 QCh 掺入纳米纤维垫中会导致 HeLa 细胞活力显著降低。研究发现，含 DOX 垫在 6 小时时的抗肿瘤功效高于游离 DOX。SEM、TEM 和荧光显微镜观察证实，基于 QCh 和含 DOX 的纤维垫的抗肿瘤作用主要归因于诱导 HeLa 细胞凋亡。
