含有季铵化壳聚糖和聚乳酸的静电纺纳米纤维垫具有体外抗 HeLa 细胞肿瘤活性。

Electrospun nanofibrous mats containing quaternized chitosan and polylactide with in vitro antitumor activity against HeLa cells.

机构信息

Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St. bl. 103A, BG-1113 Sofia, Bulgaria.

出版信息

Biomacromolecules. 2010 Jun 14;11(6):1633-45. doi: 10.1021/bm100285n.

DOI:10.1021/bm100285n

PMID:20469930

Abstract

Nanofibrous materials containing the antitumor drug doxorubicin hydrochloride (DOX) were easily prepared using a one-step method by electrospinning of DOX/poly(L-lactide-co-D,L-lactide) (coPLA) and DOX/quaternized chitosan (QCh)/coPLA solutions. The pristine and DOX-containing mats were characterized by ATR-FTIR and X-ray photoelectron spectroscopy (XPS). The release rate of DOX from the prepared fibers increased with the increase in DOX content. The DOX release process was diffusion-controlled. MTT cell viability studies revealed that incorporation of DOX and QCh in the nanofibrous mats led to a significant reduction in the HeLa cells viability. It was found, that the antitumor efficacy of the DOX-containing mats at 6 h was higher than that of the free DOX. SEM, TEM, and fluorescence microscopic observations confirmed that the antitumor effect of QCh-based and DOX-containing fibrous mats was mainly due to induction of apoptosis in the HeLa cells.

摘要

载盐酸多柔比星（DOX）的纳米纤维材料可通过静电纺丝一步法轻易制备，该方法使用 DOX/聚（L-丙交酯-co-D,L-丙交酯）（coPLA）和 DOX/季铵化壳聚糖（QCh）/coPLA 溶液。原纤维和含 DOX 的垫子通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）和 X 射线光电子能谱（XPS）进行了表征。从制备的纤维中释放 DOX 的速率随 DOX 含量的增加而增加。DOX 的释放过程为扩散控制。MTT 细胞活力研究表明，将 DOX 和 QCh 掺入纳米纤维垫中会导致 HeLa 细胞活力显著降低。研究发现，含 DOX 垫在 6 小时时的抗肿瘤功效高于游离 DOX。SEM、TEM 和荧光显微镜观察证实，基于 QCh 和含 DOX 的纤维垫的抗肿瘤作用主要归因于诱导 HeLa 细胞凋亡。