Department of Public Health, Faculty of Medicine, University of Rome Tor Vergata, Rome, Italy.
J Virol Methods. 2012 Dec;186(1-2):198-202. doi: 10.1016/j.jviromet.2012.06.007. Epub 2012 Jun 20.
Mussels (Mytilus galloprovincialis), collected from a harvesting area approved by European Community Regulation, were transplanted to four polluted sites located in the Northwestern Mediterranean area (Tuscany). They were used as bio-monitors to test the quality of the marine water pollution. At different times after the transplantation, mussels were withdrawn and tested for presence of phages and enteric viruses by molecular tests. 52.4% of the transplanted mussel samples were positive for at least one enteric virus. Hepatitis A virus (HAV) was identified in each site (17/37; 45.9%). Three samples were positive for hepatitis E virus (HEV) (8.1%) and two (5.4%) for norovirus (NoV) genogroup I. Coliphages and RYC 2056 phages were detected in all sites, while HSP 40 phages were detected in three sites. Results demonstrate the ability of transplanted mussels in accumulating and retaining different species of enteric microorganisms. Their utility as bio-monitor organisms enables testing for viral marine pollution.
贻贝（Mytilus galloprovincialis）来自于经欧洲共同体法规批准的捕捞区，被移植到位于西北地中海地区（托斯卡纳）的四个污染地点。它们被用作生物监测器，以测试海洋水污染的质量。在移植后的不同时间，取出贻贝并通过分子测试检测噬菌体和肠道病毒的存在。至少有一种肠道病毒的移植贻贝样本阳性率为 52.4%。甲型肝炎病毒 (HAV) 在每个地点均被检出（37 个样本中的 17 个；45.9%）。有 3 个样本检测到戊型肝炎病毒 (HEV)（8.1%），2 个样本检测到诺如病毒 (NoV) 基因群 I（5.4%）。在所有地点都检测到了肠噬菌体和 RYC 2056 噬菌体，而 HSP 40 噬菌体在三个地点都有检测到。研究结果表明，移植贻贝具有积累和保留不同种类肠道微生物的能力。它们作为生物监测器的用途可以用于测试海洋病毒污染。
