Plant Protection Department, University of Tabriz, Tabriz, Iran.
Arch Virol. 2012 Oct;157(10):1919-29. doi: 10.1007/s00705-012-1381-0. Epub 2012 Jun 24.
Population genetic analysis of grapevine fanleaf virus (GFLV) was done on the basis of the virus movement protein (MP) gene sequences from the isolates detected and identified in this study and those of all previously reported GFLV strains/isolates. These revealed that the GFLV populations of Iran and Slovenia were highly distinct, whereas those of France, Germany, Italy and the USA were composed of multiple lineages. All populations were significantly differentiated from each other. However, two GFLV isolates from Tunisia, the only recorded GFLVs from that country, were not statistically distinct from the French, German and Italian populations. The ratio of non-synonymous nucleotide diversity to synonymous nucleotide diversity (Pi(a)/Pi(s)) was less than 1, suggesting that the MP gene has been under purifying selection. The neutrality tests were indicative of a balancing selection that is operating within Iranian and USA GFLV isolates, but they show a purifying selection within the other populations. Eleven recombination events were detected in a total of 50 isolates from France, Germany, Iran, Italy, Slovenia and the USA. The results from the recombination analysis were in agreement with those of the phylogenetic analysis. This study suggests that diversity among GFLV geographical populations resulted from possible host adaptation, recombination and founder effects.
对本研究中检测和鉴定的葡萄扇叶病毒（GFLV）分离株和所有先前报道的 GFLV 株/分离物的病毒运动蛋白（MP）基因序列进行了种群遗传分析。结果表明，伊朗和斯洛文尼亚的 GFLV 种群高度不同，而法国、德国、意大利和美国的种群则由多个谱系组成。所有种群之间均存在显著差异。然而，来自突尼斯的两个 GFLV 分离物，是该国唯一记录的 GFLV，与法国、德国和意大利种群在统计学上没有明显区别。非同义核苷酸多样性与同义核苷酸多样性的比值（Pi(a)/Pi(s)）小于 1，表明 MP 基因受到纯化选择。中性检验表明，在伊朗和美国的 GFLV 分离物中存在平衡选择，但在其他种群中则表现出纯化选择。在来自法国、德国、伊朗、意大利、斯洛文尼亚和美国的总共 50 个分离物中检测到 11 个重组事件。重组分析的结果与系统发育分析的结果一致。本研究表明，GFLV 地理种群之间的多样性可能是由于宿主适应性、重组和奠基者效应造成的。
