Zhou Jun, Fan Xudong, Dong Yafeng, Zhang Zun Ping, Ren Fang, Hu Guojun
National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, 125100, Liaoning, China.
Arch Virol. 2015 Nov;160(11):2661-7. doi: 10.1007/s00705-015-2566-0. Epub 2015 Aug 12.
To investigate the prevalence and genetic variation of grapevine fanleaf virus (GFLV) in China, 142 grapevine samples from 13 provinces and regions were tested using DAS-ELISA, RT-PCR, and nested RT-PCR. Of the samples, 38% tested positive for GFLV by DAS-ELISA, and 26.8% tested positive by RT-PCR and nested RT-PCR. Movement protein (MP) and coat protein (CP) gene PCR products were cloned and sequenced. The MP or CP nucleotide and protein sequences shared identities that ranged from 94.9% to 100%. Phylogenetic analysis revealed that Chinese GFLV isolates obtained in this study were distinct from the isolates reported in GenBank.
为调查中国葡萄扇叶病毒（GFLV）的流行情况和遗传变异，采用双抗体夹心酶联免疫吸附测定（DAS-ELISA）、逆转录聚合酶链反应（RT-PCR）和巢式逆转录聚合酶链反应，对来自13个省和地区的142个葡萄样本进行了检测。在这些样本中，38%通过DAS-ELISA检测为GFLV阳性，26.8%通过RT-PCR和巢式RT-PCR检测为阳性。对运动蛋白（MP）和外壳蛋白（CP）基因的聚合酶链反应产物进行了克隆和测序。MP或CP的核苷酸和蛋白质序列的同源性在94.9%至100%之间。系统发育分析表明，本研究中获得的中国GFLV分离株与GenBank中报道的分离株不同。
