Newkirk D R, Righter H F, Schenck F J, Okrasinski J L, Barnes C J
Food and Drug Administration, Division of Chemistry, Rockville, MD 20857.
J Assoc Off Anal Chem. 1990 Sep-Oct;73(5):702-4.
A gas chromatographic method for determination of 2-hydroxymethyl-1-methyl-5-nitroimidazole (DMZOH), the hydroxy metabolite of dimetridazole, in swime muscle has been developed. The method uses cleanup steps similar to those of an earlier polarographic method. The present method is capable of quantitating levels as low as 2 ppb and detecting less than 1 ppb. Recoveries from 30 control tissues spiked at 1, 2, or 4 ppb averaged 80.4%. Performance of the method in incurred tissue was documented and limited data on the depletion of the metabolite in muscle were generated. The muscle of swine given 150 ppm dimetridazole in feed for 14 days contained less than 1 ppb DMZOH at 12 h withdrawal time.
已开发出一种气相色谱法，用于测定游泳肌肉中二甲硝唑的羟基代谢物2-羟甲基-1-甲基-5-硝基咪唑（DMZOH）。该方法采用的净化步骤与早期极谱法类似。本方法能够定量低至2 ppb的水平，并检测出低于1 ppb的含量。对30个添加了1、2或4 ppb的对照组织的回收率平均为80.4%。记录了该方法在实际样本组织中的性能，并生成了关于肌肉中代谢物消耗的有限数据。在饲料中添加150 ppm二甲硝唑14天的猪，在停药12小时后，其肌肉中DMZOH含量低于1 ppb。
