LeVan L W, Barnes C J
Hazleton Laboratories America, Inc., Chemical and BioMedical Sciences Division, Madison, WI 53704.
J Assoc Off Anal Chem. 1991 May-Jun;74(3):487-93.
A liquid chromatographic method for determination of thiabendazole, 5-hydroxythiabendazole, oxfendazole, mebendazole (MBZ), and fenbendazole (FBZ) in cattle liver and muscle was collaboratively studied in 7 laboratories in 1986. For blind fortified samples containing 800 ppb FBZ, average recovery and relative standard deviations for repeatability and reproducibility (RSDr and RSDR) based on results from 6 of the participating laboratories were 83%, 12.7%, and 14.0%, respectively. Recoveries of FBZ from incurred liver samples were more variable. Recoveries of MBZ from livers fortified at the 100 ppb level were encouraging; however, the drug levels were too low in the incurred samples used for MBZ studies. Except for FBZ and MBZ in liver, the study data were not satisfactory. The method has been adopted official first action by AOAC for determination of 800-1600 ppb fenbendazole in liver. The analysis should be repeated using a smaller sample size when initial analyses show levels greater than 1600 ppb FBZ.
1986年，7个实验室合作研究了一种用于测定牛肝和肌肉中噻苯达唑、5-羟基噻苯达唑、奥芬达唑、甲苯达唑（MBZ）和芬苯达唑（FBZ）的液相色谱法。对于含有800 ppb FBZ的加标盲样，根据6个参与实验室的结果，重复性和再现性的平均回收率及相对标准偏差（RSDr和RSDR）分别为83%、12.7%和14.0%。从实际污染的肝脏样品中回收FBZ的情况变化更大。在100 ppb水平加标的肝脏样品中，MBZ的回收率令人鼓舞；然而，用于MBZ研究的实际污染样品中的药物水平过低。除了肝脏中的FBZ和MBZ外，研究数据并不令人满意。该方法已被AOAC作为首次官方采用方法用于测定肝脏中800 - 1600 ppb的芬苯达唑。当初始分析显示FBZ水平大于1600 ppb时，应使用更小的样品量重复分析。
