Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
J Chem Ecol. 2012 Aug;38(8):1017-30. doi: 10.1007/s10886-012-0154-2. Epub 2012 Jun 23.
The Palearctic planthopper Hyalesthes obsoletus is the natural vector of the grapevine yellow disease Bois noir. Grapevine is an occasional host plant of this polyphagous planthopper. To deepen our knowledge of the role of plant volatile organic compounds for H. obsoletus host plant searching, we carried out behavioral, morphological, and electrophysiological studies. We tested the attraction of H. obsoletus to nettle, field bindweed, hedge bindweed, chaste tree, and grapevine by using a Y-shaped olfactometer. The results showed a significant attraction of male H. obsoletus to chaste tree, and of the females to nettle. Male H. obsoletus were repelled by odor from hedge bindweed. Ultrastructural studies of the antennae showed at least two types of olfactory sensilla at the antennal pedicel: plaque organs and trichoid sensilla. Volatile organic compounds from nettle and chaste tree were collected, and the extracts were analyzed by coupling gas-chromatography to both mass-spectrometry and electroantennography. The volatile organic compounds that elicited electrophysiological responses in male and female antennae were identified. These findings are discussed with respect to behavior of H. obsoletus males and females in the field.
东亚飞蝗 Hyalesthes obsoletus 是葡萄黄化病 Bois noir 的天然载体。葡萄是这种多食性飞蝗的偶然寄主植物。为了更深入地了解植物挥发性有机化合物在东亚飞蝗寻找寄主植物中的作用，我们进行了行为、形态和电生理研究。我们使用 Y 型嗅觉仪测试了东亚飞蝗对荨麻、田旋花、葎草、贞女树和葡萄的吸引力。结果表明，雄性东亚飞蝗对贞女树，雌性东亚飞蝗对荨麻有明显的吸引力。雄性东亚飞蝗则被葎草的气味排斥。触角的超微结构研究表明，触角柄上至少有两种类型的嗅觉感受器：斑状器官和毛形感器。收集了荨麻和贞女树的挥发性有机化合物，并通过气相色谱-质谱联用和触角电生理学分析了提取物。鉴定出了在雌雄触角上引起电生理反应的挥发性有机化合物。这些发现与东亚飞蝗在野外的雌雄行为有关。
